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Anti-inflammatory activities of isopimara-8(9),15-diene diterpenoids and mode of action of kaempulchraols B–D from Kaempferia pulchra rhizomes

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Abstract

Kaempulchraols B–D (2–4), isopimara-8(9),15-diene diterpenoids isolated from Kaempferia pulchra rhizomes collected in Myanmar, were identified as potent NF-κB inhibitors. These compounds were also effective as NO inhibitory agents, with IC50 values of 47.69, 44.97, and 38.17 μM, respectively, without showing any cytotoxicity against LPS-induced RAW264.7 cells. Investigations of the mechanisms of action of 2–4 revealed that they inhibit the NF-κB-mediated transactivation of a luciferase reporter gene, IL-6 production, and COX-2 expression, with an effective dose of 25 μM. Thus, isopimarane diterpenoids are suggested to be potent inhibitors of NF-κB pathways and could be further explored as potential anti-inflammatory lead compounds.

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Acknowledgements

This work was supported in part by Grants-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology, Japan (JSPS KAKENHI Grants JP17H02203, 17H06398, 19K23794, and 19H04649), the JSPS RONPAKU Program (R11815), and the Takahashi Industrial and Economic Research Foundation (H.M.).

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Authors and Affiliations

  1. Institute of Natural Medicine, University of Toyama, 2630-Sugitani, Toyama, 930-0194, Japan

    Nwet Nwet Win, Besse Hardianti, Yoshihiro Hayakawa & Hiroyuki Morita

  2. Sekolah Tinggi Ilmu Farmasi Makassar, Perintis Kemerdekaan Street KM 13.7, Makassar, 90242, Indonesia

    Besse Hardianti

  3. Department of Chemistry, University of Yangon, Yangon, 11041, Myanmar

    Hla Ngwe

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  1. Nwet Nwet Win
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  2. Besse Hardianti
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  3. Hla Ngwe
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  4. Yoshihiro Hayakawa
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  5. Hiroyuki Morita
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Correspondence to Yoshihiro Hayakawa or Hiroyuki Morita.

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Win, N.N., Hardianti, B., Ngwe, H. et al. Anti-inflammatory activities of isopimara-8(9),15-diene diterpenoids and mode of action of kaempulchraols B–D from Kaempferia pulchra rhizomes. J Nat Med 74, 487–494 (2020). https://doi.org/10.1007/s11418-020-01389-7

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  • DOI: https://doi.org/10.1007/s11418-020-01389-7

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