Rhamnan sulfate extracted from Monostroma nitidum attenuates blood coagulation and inflammation of vascular endothelial cells


Rhamnan sulfate (RS) is a polysaccharide with a rhamnose backbone isolated from Monostroma nitidum. Like heparin, it exerts anticoagulant activity in the presence of antithrombin. Endothelial cells facilitate the crosstalk between blood coagulation and vascular inflammation. In this study, we compared the effect of RS with that of heparin on blood coagulation and vascular endothelial cells in the presence or absence of inflammatory factors, using human umbilical vein endothelial cells. We found that RS significantly enhances inhibition of thrombin and factor Xa in the presence of antithrombin as well as heparin, and that RS inhibits tissue factor expression and von Willebrand factor release from the endothelial cells treated with or without lipopolysaccharide, tumor necrosis factor-α, or thrombin. Heparin did not show any effects on endothelial cell inflammation. Our findings suggest that RS, like heparin, is an antithrombin-dependent anticoagulant and, unlike heparin, is a potent anti-inflammatory agent acting on vascular endothelial cells.

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Activated partial thromboplastin time


Human umbilical vein endothelial cells




Prothrombin time


Rhamnan sulfate


Tissue factor


Tumor necrosis factor


Von Willebrand factor


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This work was supported in part by a Grant-in-Aid for challenging Exploratory Research (Grant number 18659280), a Grant-in-Aid for Scientific Research (B) (Grant number 21309292), and a Grant-in-Aid for Scientific Research (C) (Grant numbers 25460396 and 16K08633) from the Japan Society for the Promotion of Science.

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Correspondence to Koji Suzuki.

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This study was performed as a collaborative investigation funded by the Konan Chemical Manufacturing Co. Ltd. The corresponding author had full access to all data in the study and had final responsibility for the decision to submit for publication.

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Okamoto, T., Akita, N., Terasawa, M. et al. Rhamnan sulfate extracted from Monostroma nitidum attenuates blood coagulation and inflammation of vascular endothelial cells. J Nat Med 73, 614–619 (2019). https://doi.org/10.1007/s11418-019-01289-5

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  • Monostroma nitidum
  • Seaweed
  • Rhamnan sulfate
  • Anti-coagulation
  • Anti-inflammation
  • Vascular endothelium