Abstract
Podolactones are a class of structural diverse diterpenoid lactones, mainly isolated from the Podocarpus species. Several bioactivities have been disclosed for podolactones, including cytotoxicity and anti-atherosclerosis. In this study, the seeds of P. nagi were isolated by comprehensive chromatographic methods to obtain three new podolatones, named nagilactone B 1-O-β-d-glucoside (1), nagilactone N3 3-O-β-d-glucoside (2), and 2-epinagilactone B (3), as well as a known compound, nagilactone B (4). Their structures were determined by analyses of NMR and HRESIMS data. Compounds 1 and 2 significantly inhibited nitric oxide (NO) production on LPS-stimulated RAW264.7 macrophages, with IC50 values of 0.18 ± 0.04 and 0.53 ± 0.03 μM, respectively. Indomethacin (IC50 4.21 ± 0.32 μM) was used as a positive control. Compound 1 suppressed the expression of inducible NO synthase (iNOS) in a concentration-dependent manner, mediating through inhibiting nuclear factor-κB (NF-κB) activity. This is the first report regarding the anti-inflammatory effect of podolactones, which could be potential anti-inflammatory agents.
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Financial support by Science and Technology Development Fund, Macao S.A.R (FDCT 102/2017/A), the Research Fund of University of Macau (MYRG2017-00109-ICMS), and the State Key Laboratory of Drug Research (SIMM1803KF-16) are gratefully acknowledged.
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Feng, ZL., Zhang, T., Liu, JX. et al. New podolactones from the seeds of Podocarpus nagi and their anti-inflammatory effect. J Nat Med 72, 882–889 (2018). https://doi.org/10.1007/s11418-018-1219-5
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DOI: https://doi.org/10.1007/s11418-018-1219-5