Methylquercetins stimulate melanin biosynthesis in a three-dimensional skin model
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In a previous study, we found that both synthetic 3-O-methylquercetin (3MQ) and 3,4′,7-O-trimethylquercetin (34′7TMQ) increased extracellular melanin content. 34′7TMQ increased the activity of melanogenic enzymes by stimulating the p38 pathway and the expression of microphthalmia-associated transcription factor (MITF). In contrast, 3MQ increased the activity of melanogenic enzymes without the involvement of MITF, which suggests that 3MQ inhibits the degradation of melanogenic enzymes. In the present study, we investigated the effects of 3MQ and 34′7TMQ on melanogenesis in normal human melanocytes and using a commercial three-dimensional (3D) skin model system. Both 3MQ and 34′7TMQ elongated the dendrites of normal human melanocytes from a Caucasian donor, but did not stimulate melanogenesis in the melanocytes. In the 3D skin model, which included melanocytes from an Asian donor, 3MQ and 34′7TMQ increased and elongated the melanocytes and showed a tendency to stimulate melanogenesis. These results suggest that 3MQ and 34′7TMQ could be put to practical use in skin care products and agents aimed at preventing hair graying.
KeywordsB16 melanoma cells Three-dimensional skin model Normal human melanocyte Methylquercetin
This work was supported by a Research Fellowship from the Japan Society for the Promotion of Science (JSPS).
- 3.Pérez-Sánchez A, Barrajón-Catalán E, Herranz-López M et al (2016) Lemon balm extract (Melissa officinalis, L.) promotes melanogenesis and prevents UVB-induced oxidative stress and DNA damage in a skin cell model. J Dermatol Sci 84:169–177. https://doi.org/10.1016/J.JDERMSCI.2016.08.004 CrossRefGoogle Scholar
- 12.Jang JY, Kim HN, Kim YR et al (2011) Partially purified components of Nardostachys chinensis suppress melanin synthesis through ERK and Akt signaling pathway with cAMP down-regulation in B16F10 cells. J Ethnopharmacol 137:1207–1214. https://doi.org/10.1016/j.jep.2011.07.047 CrossRefPubMedPubMedCentralGoogle Scholar
- 19.Yamauchi K, Mitsunaga T, Itakura Y, Batubara I (2015) Extracellular melanogenesis inhibitory activity and the structure–activity relationships of ugonins from Helminthostachys zeylanica roots. Fitoterapia 104:69–74. https://doi.org/10.1016/j.fitote.2015.05.006 CrossRefPubMedPubMedCentralGoogle Scholar
- 28.Yamauchi K, Mitsunaga T, Inagaki M, Suzuki T (2014) Synthesized quercetin derivatives stimulate melanogenesis in B16 melanoma cells by influencing the expression of melanin biosynthesis proteins MITF and p38 MAPK. Bioorgan Med Chem 22:3331–3340. https://doi.org/10.1016/j.bmc.2014.04.053 CrossRefGoogle Scholar
- 40.Hussein A, Al-Wadei N, Takahashi T, Schuller HM (2005) Theophylline stimulates cAMP-mediated signaling associated with growth regulation in human cells from pulmonary adenocarcinoma and small airway epithelia. Int J Oncol 27:155–160Google Scholar