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Dehydropropylpantothenamide isolated by a co-culture of Nocardia tenerifensis IFM 10554T in the presence of animal cells

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Abstract

A new amide, named dehydropropylpantothenamide (1), was obtained by a co-culture of Nocardia tenerifensis IFM 10554T in the presence of the mouse macrophage-like cell line J774.1 in modified Czapek-Dox (mCD) medium. Compound 1 was synthesized from d-pantothenic acid calcium salt in 6 steps. The absolute configuration of natural compound 1 was determined by comparisons of the optical rotation and CD spectra of synthetic 1. In the present study, a new method for producing secondary metabolites was demonstrated using a “co-culture” in which the genus Nocardia was cultured in the presence of an animal cell line.

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Acknowledgments

This study was supported by JSPS KAKENHI Grant Number 17H03992 and the Strategic Priority Research Promotion Program of Chiba University “Phytochemical Plant Molecular Sciences”.

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Authors and Affiliations

  1. Graduate School of Pharmaceutical Sciences, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba, 260-8675, Japan

    Yasumasa Hara, Midori A. Arai, Naoki Ishikawa & Masami Ishibashi

  2. Medical Mycology Research Center, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba, 260-8673, Japan

    Kanae Sakai, Tohru Gonoi & Takashi Yaguchi

Authors
  1. Yasumasa Hara
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  2. Midori A. Arai
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  3. Kanae Sakai
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  4. Naoki Ishikawa
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  5. Tohru Gonoi
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  6. Takashi Yaguchi
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  7. Masami Ishibashi
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Correspondence to Masami Ishibashi.

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Hara, Y., Arai, M.A., Sakai, K. et al. Dehydropropylpantothenamide isolated by a co-culture of Nocardia tenerifensis IFM 10554T in the presence of animal cells. J Nat Med 72, 280–289 (2018). https://doi.org/10.1007/s11418-017-1161-y

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  • DOI: https://doi.org/10.1007/s11418-017-1161-y

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