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AU-1 from Agavaceae plants downregulates the expression of glycolytic enzyme phosphoglycerate mutase

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Abstract

The spirostanol saponin AU-1 from Agavaceae plants stimulates the expression of the glycolytic enzyme phosphoglycerate mutase (PGAM) in ACHN cells. We hypothesized that this may arise from the downregulation of the NAD+-dependent deacetylase SIRT1. In this article, we showed that, unlike in renal adenocarcinoma cells, AU-1 does not affect the expression of SIRT1 in the normal renal cell-derived cell line HK-2. Consistent with the lack of a downregulation of SIRT1, AU-1 did not upregulate, but rather decreased PGAM expression. Moreover, AU-1 inhibited the increase in PGAM levels that results from the knock-down of SIRT1. Our results suggest that AU-1 may prevent carcinogenesis caused by increased cellular PGAM.

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Acknowledgements

We thank Ken Ando for their helpful advice and discussions. This work was supported in part by a grant from the Japan Private School Promotion Foundation.

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Authors and Affiliations

  1. Department of Hygiene and Health Sciences, School of Pharamacy, Tokyo University of Pharmacy and Life Sciences, Horinouchi 1432-1, Hachioji, Tokyo, 192-0392, Japan

    Tomofumi Fujino, Harutaka Ichikawa, Toshiyuki Oshima & Makio Hayakawa

  2. Department of Medicinal Pharmacognosy, School of Pharamacy, Tokyo University of Pharmacy and Life Sciences, Horinouchi 1432-1, Hachioji, Tokyo, 192-0392, Japan

    Akihito Yokosuka & Yoshihiro Mimaki

Authors
  1. Tomofumi Fujino
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  2. Akihito Yokosuka
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  3. Harutaka Ichikawa
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  4. Toshiyuki Oshima
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  5. Yoshihiro Mimaki
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  6. Makio Hayakawa
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Correspondence to Tomofumi Fujino.

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Fujino, T., Yokosuka, A., Ichikawa, H. et al. AU-1 from Agavaceae plants downregulates the expression of glycolytic enzyme phosphoglycerate mutase. J Nat Med 72, 342–346 (2018). https://doi.org/10.1007/s11418-017-1154-x

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  • DOI: https://doi.org/10.1007/s11418-017-1154-x

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