Phytochemical profile and angiotensin I converting enzyme (ACE) inhibitory activity of Limonium michelsonii Lincz
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Members of the genus Limonium are widely used as medicinal herbs due to their health-promoting effects, such as an ability to improve blood circulation by inhibiting angiotensin I converting enzyme (ACE). While the potential of L. michelsonii Lincz. (a medicinal plant endemic to Kazakhstan) to inhibit ACE has been demonstrated, the inhibitory activities of its secondary metabolites have not been explored. In this work, the principal phenolic compounds (1–20) among these metabolites were isolated to determine the components responsible for ACE inhibition. The natural abundances of the active constituents within the target plant were characterized by UPLC-Q-TOF/MS analysis. All of the isolated compounds except for gallates 10–12 were found to significantly inhibit ACE, with IC50 values of between 7.1 and 138.4 μM. Unexpectedly, the flavonol glycosides 16–20 were observed to be more potent than the corresponding aglycones 4 and 5. For example, quercetin (4) had IC50 = 30.3 μM, whereas its glycosides (16, 17) had IC50 = 10.2 and 14.5 μM, respectively. A similar trend was observed for myricetin (5) and its glycosides (18–20). In a kinetic study, the flavonols 3–5 and 16–20 and the dihydroflavonols 8 and 9 behaved as competitive inhibitors, whereas other flavones (1, 2, 13–15) and flavanones (6, 7) performed noncompetitive inhibition.
KeywordsLimonium michelsonii Lincz. Angiotensin I converting enzyme Phenolic metabolites Competitive inhibitors
Angiotensin I converting enzyme
Inhibitor concentration that produces a 50% decrease in activity
This work was done with research funds from the Ministry of Agriculture, Food and Rural Affairs (No. 315032-04-2-SB010) and the Next-Generation BioGreen 21 program, Rural Development Administration (SSAC, No. PJ01107001), Republic of Korea. The BK21 PLUS program supported scholarships for senior researchers and all other students.
Compliance with ethical standards
Conflict of interest
The authors declare no conflict of interest.
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