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Journal of Natural Medicines

, Volume 71, Issue 4, pp 606–616 | Cite as

Triterpenoid saponins from Polaskia chichipe Backbg. and their inhibitory or promotional effects on the melanogenesis of B16 melanoma cells

  • Koji Fujihara
  • Kunio Takahashi
  • Kiyotaka Koyama
  • Kaoru Kinoshita
Original Paper
  • 314 Downloads

Abstract

Five new oleanane-type saponins 15 together with a known saponin 6 and a steroidal glycoside 7 were isolated from Polaskia chichipe Backbg., and their structures were determined from their 1D and 2D NMR and HRFABMS spectral data. The six isolated saponins 16 were tested for their effects on the melanogenesis of B16 melanoma 4A5 cells. Compound 1 exerted an inhibitory effect at 100 μM whereas compound 3 promoted melanogenesis at the same concentration, even though these two compounds contain the same aglycon structure. The dose-dependent activities of compounds 1 and 3 on melanin synthesis were investigated.

Keywords

Cactus Polaskia chichipe Saponins Melanin B16 Chichipegenin Oleanolic acid Chichipenoside 

Notes

Acknowledgements

We would like to thank Miss Tamami Koseki (MS spectra) and Mrs. Shoko Yamada (NMR spectra) from the instrumental analysis center in Meiji Pharmaceutical University for measuring the MS and NMR spectra.

Supplementary material

11418_2017_1082_MOESM1_ESM.pdf (8.3 mb)
Supplementary material 1 (PDF 8451 kb)

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Copyright information

© The Japanese Society of Pharmacognosy and Springer Japan 2017

Authors and Affiliations

  • Koji Fujihara
    • 1
  • Kunio Takahashi
    • 1
  • Kiyotaka Koyama
    • 1
  • Kaoru Kinoshita
    • 1
  1. 1.Department of Pharmacognosy and PhytochemistryMeiji Pharmaceutical UniversityTokyoJapan

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