Journal of Natural Medicines

, Volume 71, Issue 1, pp 257–264 | Cite as

Gastroprotective effect of palmatine against acetic acid-induced gastric ulcers in rats

  • Ling Wang
  • Xue Wang
  • Su-Li Zhang
  • Xiao-Ming Zhu
  • Yan-Qi Liu
  • Zi-Jing Song
  • Wen-Juan Du
  • Jun Ji
  • Cheng-Long Cui
  • Xin He
  • Chun-Feng Zhang
  • Chang-Run Guo
  • Chong-Zhi Wang
  • Chun-Su Yuan
Original Paper

Abstract

Gastric ulcers are one of the most common gastrointestinal disorders. The aim of this study was to investigate the gastroprotective activity and possible underlying mechanisms of palmatine against acetic acid-induced gastric ulcers in rats. Palmatine was administered orally for 7 consecutive days to treat ulcers. The ulcer area, ulcer inhibition rate, histological section, platelet-activating factor (PAF) level in serum, prostaglandin E2 (PGE2) level in gastric tissue, 5-hydroxytryptamine (5-HT) level in the brain and norepinephrine (NE) level in the adrenal glands were analyzed. Histological results showed that the ulcer areas were significantly decreased by both doses of palmatine (10 and 20 mg/kg/day) compared with the model group, and the ulcer inhibition rates were 51.42% and 60.92%, respectively. Palmatine treatment markedly increased the level of PGE2 and decreased PAF, compared with the model group; however, it had no significant effect on 5-HT and NE levels. The results indicated that palmatine may exert a gastroprotective effect against gastric ulcers, and the mechanisms might be associated with the anti-inflammatory status and the protection of gastric mucosa via increasing PGE2 and decreasing PAF rather than neurohumoral regulation through 5-HT and NE. Thus, palmatine is a potential drug for treatment of gastric ulcers.

Keywords

Palmatine Gastroprotective action Acetic acid-induced gastric ulcer Anti-inflammation 

Abbreviations

PAF

Platelet-activating factor

PGE2

Prostaglandin E2

5-HT

5-Hydroxytryptamine

NE

Norepinephrine

CMC-Na

Sodium carboxymethylcellulose

ELISA

Enzyme-linked immunosorbent assay

Sop

Sham-operated group

Mod

Model group

Omp

Omeprazole group

Wkl

Weikangling capsule

LP

Low-dose palmatine group

HP

High-dose palmatine group

H&E

Hematoxylin-eosin

IPP

Image-Pro Plus

SEM

Standard error of measurement

ANOVA

One-way analysis of variance

AA

Arachidonic acid

PLA2

Phospholipase A2

COX-2

Cyclooxygenase 2

PGES

Prostaglandin E synthase

HPA

Hypothalamic pituitary adrenal

Notes

Acknowledgements

This work was supported by the National Natural Science Foundation of China (81573565), the College Students Innovation Project for the R&D of Novel Drugs (J1030830) and National Training Programs of Innovation and Entrepreneurship for College Students.

Compliances with ethical standards

Conflict of interest

We have no conflict of interest in this research.

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Copyright information

© The Japanese Society of Pharmacognosy and Springer Japan 2016

Authors and Affiliations

  • Ling Wang
    • 1
  • Xue Wang
    • 1
  • Su-Li Zhang
    • 1
  • Xiao-Ming Zhu
    • 1
  • Yan-Qi Liu
    • 1
  • Zi-Jing Song
    • 1
  • Wen-Juan Du
    • 1
  • Jun Ji
    • 1
  • Cheng-Long Cui
    • 1
  • Xin He
    • 2
  • Chun-Feng Zhang
    • 1
    • 3
  • Chang-Run Guo
    • 1
  • Chong-Zhi Wang
    • 3
  • Chun-Su Yuan
    • 3
  1. 1.State Key Laboratory of Natural MedicinesChina Pharmaceutical UniversityNanjingChina
  2. 2.School of PharmacyNanjing University of Chinese MedicineNanjingChina
  3. 3.Department of Anesthesia and Critical CareTang Center of Herbal Medicine Research, University of ChicagoChicagoUSA

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