Skip to main content
SpringerLink
Log in

Efficacy of Kaempferia parviflora in a mouse model of obesity-induced dermatopathy

  • Original Paper
  • Published:
Journal of Natural Medicines Aims and scope Submit manuscript

Abstract

Obesity results from excessive energy intake and physical inactivity, and predisposes one to various diseases. One of these reasons is that enlargement of adipocytes raises the lipid metabolic abnormalities that affect various organs. The skin is one such organ, and it has been reported that subcutaneous adipocyte cells secrete various factors and these factors are involved in reduction of dermal collagen fibers and fragility of the skin in obesity. The present study explored the efficacy of Kaempferia parviflora (KP) in preventing obesity-induced dermatopathy. We used Tsumura Suzuki obese diabetes (TSOD) mice as an obesity model. TSOD mice were fed a standard diet (MF) mixed with either an ethanol extract from KP (KPE), polymethoxyflavonoid-rich extract from KP (PMF), or polymethoxyflavonoid-poor extract from KP (X). We then evaluated the effect of these three KP fractions on aging-like skin damage induced by UVB irradiation. KPE and PMF caused a significant decrease of mouse body weight, and suppressed the increase in the thickness of the subcutaneous fat layer. In addition, KPE shifted the frequency of subcutaneous adipocyte sizes towards smaller cells possibly via its polypharmacological actions. Scanning electron microscopy revealed that the stereostructure of the collagenous fibers in the dermis was better retained in the KPE and PMF groups, in that order. These results offer the first evidence that KPE can attenuate obesity-induced dermatopathy more effectively than PMF, suggesting that KPE (or KP) might be a candidate supplement for preventing obesity-related skin disorders.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

References

  1. Ministry of Health, Labour and Welfare (2013) National health and nutrition examination survey results summary. http://www.mhlw.go.jp/file/04-Houdouhappyou-10904750-Kenkoukyoku-Gantaisakukenkouzoushinka/0000106403.pdf. Accessed 21 Dec 2015

  2. Mount P, Davies M, Choy SW, Cook N, Power D (2015) Obesity-related chronic kidney disease-the role of lipid metabolism. Metabolites 5(4):720–731

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. Ezure T, Amano S (2010) Increased subcutaneous adipose tissue impairs dermal function in diet-induced obese mice. Exp Dermatol 19(10):878–882

    Article  CAS  PubMed  Google Scholar 

  4. Ibuki A, Akase T, Nagase T, Minematsu T, Nakagami G, Horii M, Sagara H, Komeda T, Kobayashi M, Shimada T, Aburada M, Yoshimura K, Sugama J, Sanada H (2012) Skin fragility in obese diabetic mice: possible involvement of elevated oxidative stress and upregulation of matrix metalloproteinases. Exp Dermatol 21(3):178–183

    Article  CAS  PubMed  Google Scholar 

  5. Lerman OZ, Galiano RD, Armour M, Levine JP, Gurtner GC (2003) Cellular dysfunction in the diabetic fibroblast: impairment in migration, vascular endothelial growth factor production, and response to hypoxia. Am J Pathol 162(1):303–312

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  6. Akase T, Nagase T, Huang L, Ibuki A, Minematsu T, Nakagami G, Ohta Y, Shimada T, Aburada M, Sugama J, Sanada H (2012) Aging-like skin changes induced by ultraviolet irradiation in an animal model of metabolic syndrome. Biol Res Nurs 14(2):180–187

    Article  CAS  PubMed  Google Scholar 

  7. Kusirisin W, Srichairatanakool S, Lerttrakarnnon P, Lailerd N, Suttajit M, Jaikang C, Chaiyasut C (2009) Antioxidative activity, polyphenolic content and anti-glycation effect of some Thai medicinal plants traditionally used in diabetic patients. Med Chem 5(2):139–147

    Article  CAS  PubMed  Google Scholar 

  8. Sae-wong C, Tansakul P, Tewtrakul S (2009) Anti-inflammatory mechanism of Kaempferia parviflora in murine macrophage cells (RAW 264.7) and in experimental animals. J Ethnopharmacol 124(3):576–580

    Article  CAS  PubMed  Google Scholar 

  9. Rujjanawate C, Kanjanapothi D, Amornlerdpison D, Pojanagaroon S (2005) Anti-gastric ulcer effect of Kaempferia parviflora. J Ethnopharmacol 102(1):120–122

    Article  CAS  PubMed  Google Scholar 

  10. Horigome S, Yoshida I, Tsuda A, Harada T, Yamaguchi A, Yamazaki K, Inohana S, Isagawa S, Kibune N, Satoyama T, Katsuda S, Suzuki S, Watai M, Hirose N, Mitsue T, Shirakawa H, Komai M (2014) Identification and evaluation of anti-inflammatory compounds from Kaempferia parviflora. Biosci Biotechnol Biochem 78(5):851–860

    Article  CAS  PubMed  Google Scholar 

  11. Albrecht M, Frungieri MB, Kunz L, Rämsch R, Meineke V, Köhn FM, Mayerhofer A (2005) Divergent effects of the major mast cell products histamine, tryptase and TNF-alpha on human fibroblast behaviour. Cell Mol Life Sci 62(23):2867–2876

    Article  CAS  PubMed  Google Scholar 

  12. Akase T, Shimada T, Terabayashi S, Ikeya Y, Sanada H, Aburada M (2011) Antiobesity effects of Kaempferia parviflora in spontaneously obese type II diabetic mice. J Nat Med 65(1):73–80

    Article  PubMed  Google Scholar 

  13. Park JE, Pyun HB, Woo SW, Jeong JH, Hwang JK (2013) The protective effect of Kaempferia parviflora extract on UVB-induced skin photoaging in hairless mice. Photodermatol Photoimmunol Photomed 30(5):237–245

    Article  Google Scholar 

  14. Parlee SD, Lentz SI, Mori H, MacDougald OA (2014) Quantifying size and number of adipocytes in adipose tissue. Method Enzymol 537:93–122

    Article  CAS  Google Scholar 

  15. Simon BR, Learman BS, Parlee SD, Scheller EL, Mori H, Cawthorn WP, Ning X, Krishnan V, Ma YL, Tyrberg B, MacDougald OA (2014) Sweet taste receptor deficient mice have decreased adiposity and increased bone mass. PLoS One 9(1):e86454

    Article  PubMed  PubMed Central  Google Scholar 

  16. Galarraga M, Campión J, Muñoz-Barrutia A, Boqué N, Moreno H, Martínez JA, Milagro F, Ortiz-de-Solórzano C (2012) Adiposoft: automated software for the analysis of white adipose tissue cellularity in histological sections. J Lipid Res 53(12):2791–2796

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  17. Amaro-Ortiz A, Yan B, D’Orazio JA (2014) Ultraviolet radiation, aging and the skin: prevention of damage by topical cAMP manipulation. Molecules 19(5):6202–6219

    Article  PubMed  PubMed Central  Google Scholar 

  18. Geldenhuys S, Hart PH, Endersby R, Jacoby P, Feelisch M, Weller RB, Matthews V, Gorman S (2014) Ultraviolet radiation suppresses obesity and symptoms of metabolic syndrome independently of vitamin D in mice fed a high-fat diet. Diabetes 63(11):3759–3769

    Article  CAS  PubMed  Google Scholar 

  19. Horikawa T, Shimada T, Okabe Y, Kinoshita K, Koyama K, Miyamoto K, Ichinose K, Takahashi K, Aburada M (2012) Polymethoxyflavonoids from Kaempferia parviflora induce adipogenesis on 3T3-L1 preadipocytes by regulating transcription factors at an early stage of differentiation. Biol Pharm Bull 35(5):686–692

    Article  CAS  PubMed  Google Scholar 

  20. Okabe Y, Shimada T, Horikawa T, Kinoshita K, Koyama K, Ichinose K, Aburada M, Takahashi K (2014) Suppression of adipocyte hypertrophy by polymethoxyflavonoids isolated from Kaempferia parviflora. Phytomedicine 21(6):800–806

    Article  CAS  PubMed  Google Scholar 

  21. Mekjaruskul C, Jay M, Sripanidkulchai B (2012) Pharmacokinetics, bioavailability, tissue distribution, excretion, and metabolite identification of methoxyflavones in Kaempferia parviflora extract in rats. Drug Metab Dispos 40(12):2342–2353

    Article  CAS  PubMed  Google Scholar 

  22. Junqueira LC, Montes GS, Martins JE, Joazeiro PP (1983) Dermal collagen distribution. A histochemical and ultrastructural study. Histochemistry 79(3):397–403

    Article  CAS  PubMed  Google Scholar 

  23. Gesta S, Tseng YH, Kahn CR (2007) Developmental origin of fat: tracking obesity to its source. Cell 131(2):242–256

    Article  CAS  PubMed  Google Scholar 

  24. Chau YY, Bandiera R, Serrels A, Martínez-Estrada OM, Qing W, Lee M, Slight J, Thornburn A, Berry R, McHaffie S, Stimson RH, Walker BR, Chapuli RM, Schedl A, Hastie N (2014) Visceral and subcutaneous fat have different origins and evidence supports a mesothelial source. Nat Cell Biol 16(4):367–375

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  25. Kobayashi H, Horiguchi-Babamoto E, Suzuki M, Makihara H, Tomozawa H, Tsubata M, Shimada T, Sugiyama K, Aburada M (2016) Effects of ethyl acetate extract of Kaempferia parviflora on brown adipose tissue. J Nat Med 70(1):54–61

    Article  CAS  PubMed  Google Scholar 

  26. Labbé SM, Caron A, Lanfray D, Monge-Rofarello B, Bartness TJ, Richard D (2015) Hypothalamic control of brown adipose tissue thermogenesis. Front Syst Neurosci 9:150

    Article  PubMed  PubMed Central  Google Scholar 

Download references

Acknowledgments

This work was supported by a grant-in-aid for Scientific Research from MEXT (Ministry of Education Culture, Sports, Science and Technology) (No. 1224593252).

Author information

Authors and Affiliations

  1. Department of Biological Science and Nursing, School of Medicine, Yokohama City University, 3-9 Fukuura, Kanazawa-ku, Yokohama, Kanagawa, 236-0004, Japan

    Moeko Hidaka, Kazumasa Horikawa, Tomoko Akase, Hiroko Makihara, Ai Ibuki & Yutaka Matsumoto

  2. Department of Molecular Pharmacology and Neurobiology, Graduate School of Medicine, Yokohama City University, 3-9 Fukuura, Kanazawa-ku, Yokohama, Kanagawa, 236-0004, Japan

    Hiroko Makihara

  3. Research and Development Division, Toyo Shinyaku Co., Ltd.,, 7-28 Yayoigaoka, Tosu, Saga, 841-0005, Japan

    Takatoshi Ogami, Hiroshi Tomozawa & Masahito Tsubata

Authors
  1. Moeko Hidaka
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Kazumasa Horikawa
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Tomoko Akase
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Hiroko Makihara
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Takatoshi Ogami
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Hiroshi Tomozawa
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Masahito Tsubata
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Ai Ibuki
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Yutaka Matsumoto
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Tomoko Akase.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary material 1 (DOCX 27 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Hidaka, M., Horikawa, K., Akase, T. et al. Efficacy of Kaempferia parviflora in a mouse model of obesity-induced dermatopathy. J Nat Med 71, 59–67 (2017). https://doi.org/10.1007/s11418-016-1027-8

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11418-016-1027-8

Keywords

Search

Navigation