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Application of a quantitative 1H-NMR method for the determination of paeonol in Moutan cortex, Hachimijiogan and Keishibukuryogan

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Abstract

Quantitative 1H-NMR (1H-qNMR) was applied to the determination of paeonol concentration in Moutan cortex, Hachimijiogan, and Keishibukuryogan. Paeonol is a major component of Moutan cortex, and its purity was calculated from the ratio of the intensity of the paeonol H-3′ signal at δ 6.41 ppm in methanol-d 4 or 6.40 ppm in methanol-d 4 + TFA-d to that of a hexamethyldisilane (HMD) signal at 0 ppm. The concentration of HMD was corrected with SI traceability by using potassium hydrogen phthalate of certified reference material grade. As a result, the paeonol content in two lots of Moutan cortex as determined by 1H-qNMR was found to be 1.59 % and 1.62 %, respectively, while the paeonol content in Hachimijiogan and Keishibukuryogan was 0.15 % and 0.22 %, respectively. The present study demonstrated that the 1H-NMR method is useful for the quantitative analysis of crude drugs and Kampo formulas.

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Acknowledgments

We thank Dr. Y. Goda of the National Institute of Health Sciences for their kind advice regarding 1H-qNMR analysis. This study was supported in part by a grant from the Japan Food Chemical Research Foundation and by a Health Labour Sciences Research Grant from the Ministry of Health, Labour and Welfare, Japan.

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Authors and Affiliations

  1. Laboratory of Pharmacognosy, College of Pharmacy, Kinjo Gakuin University, 2-1723 Omori, Moriyama-ku, Nagoya, 463-8521, Japan

    Rie Tanaka, Hikari Shibata & Akito Nagatsu

  2. National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, Tokyo, 158-8501, Japan

    Rie Tanaka, Naoki Sugimoto & Hiroshi Akiyama

Authors
  1. Rie Tanaka
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  2. Hikari Shibata
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  3. Naoki Sugimoto
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Correspondence to Akito Nagatsu.

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Tanaka, R., Shibata, H., Sugimoto, N. et al. Application of a quantitative 1H-NMR method for the determination of paeonol in Moutan cortex, Hachimijiogan and Keishibukuryogan. J Nat Med 70, 797–802 (2016). https://doi.org/10.1007/s11418-016-1003-3

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  • DOI: https://doi.org/10.1007/s11418-016-1003-3

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