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Anti-inflammatory effects of alpinone 3-acetate from Alpinia japonica seeds

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Abstract

We aimed to investigate the bioactive components of Alpinia japonica as anti-inflammatory compounds using searches of the Alpinia genus, and subsequently demonstrated that alpinone 3-acetate markedly inhibits 12-O-tetradecanoyiphorbol 13-acetate-induced inflammation in a mouse model of ear edema. To assess other bioactivities of alpinone 3-acetate, we performed translatome analyses and compared them with those of hydrocortisone. Polysome-associated mRNAs were prepared from alpinone 3-acetate- or hydrocortisone-treated and control cells from 12-O-tetradecanoyiphorbol 13-acetate-induced THP-1-derived macrophages cultured in the presence of Escherichia coli O-111 lipopolysaccharide. Subsequent microarray analysis revealed that alpinone 3-acetate and hydrocortisone upregulated and downregulated the same 155 and 41 genes, respectively. Moreover, direct comparisons of translationally regulated genes indicated 5 and 10 gene probes that were upregulated and downregulated by alpinone 3-acetate and hydrocortisone, respectively. In conclusion, assays of 12-O-tetradecanoyiphorbol 13-acetate-induced inflammation ear edema in mice and polysome profiling of alpinone 3-acetate bioactivities indicated similar medicinal possibilities to those of hydrocortisone.

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Authors and Affiliations

  1. Faculty of Pharmaceutical Sciences, Josai International University, 1 Gumyo, Togane, Chiba, 283-8555, Japan

    Tomohito Kakegawa & Aya Miyazaki

  2. School of Pharmacy, Nihon University, 7-7-1 Narashinodai, Funabashi, Chiba, 274-8555, Japan

    Ken Yasukawa

Authors
  1. Tomohito Kakegawa
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  2. Aya Miyazaki
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  3. Ken Yasukawa
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Correspondence to Tomohito Kakegawa.

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Kakegawa, T., Miyazaki, A. & Yasukawa, K. Anti-inflammatory effects of alpinone 3-acetate from Alpinia japonica seeds. J Nat Med 70, 653–660 (2016). https://doi.org/10.1007/s11418-016-0997-x

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  • DOI: https://doi.org/10.1007/s11418-016-0997-x

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