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Journal of Natural Medicines

, Volume 70, Issue 1, pp 45–53 | Cite as

Purple potato (Solanum tuberosum L.) anthocyanins attenuate alcohol-induced hepatic injury by enhancing antioxidant defense

  • Zhihui Jiang
  • Chen Chen
  • Jian Wang
  • Wenyan Xie
  • Meng Wang
  • Xinsheng Li
  • Xiaoying Zhang
Original Paper

Abstract

Alcoholic liver disease (ALD) is a serious and challenging health issue. In the past decade, natural components possessing hepatoprotective properties have gained more attention for ALD intervention. In this study, the phytochemical components of anthocyanins from purple potato were assessed using UPLC-MS/MS, and the hepatoprotective effects of purple potato anthocyanins (PPAs) were investigated in the ALD mouse model. Serum and liver biochemical parameters were determined, along with histopathological changes in liver tissue. In addition, the major contributors to alcohol-induced oxidative stress were assessed. The results indicated that the levels of aspartate transaminase and alanine transaminase were lower in the serum of the PPA-treated group than the alcohol-treated group. PPAs significantly inhibited the reduction of total cholesterol and triglycerides. Higher levels of superoxide dismutase and reduced glutathione enzymes as well as a reduction in the formation of malondialdehyde occurred in mice fed with PPAs. In addition, PPAs protected against increased alcohol-induced levels and activity of cytochrome P450 2E1 (CYP2E1), which demonstrates the effects of PPAs against alcohol-induced oxidative stress and liver injury. This study suggests that PPAs could be an effective therapeutic agent in alcohol-induced liver injuries by inhibiting CYP2E1 expression and thereby strengthening antioxidant defenses.

Keywords

Anthocyanin Hepatotoxicity Alcohol Oxidation stress CYP2E1 

Abbreviations

ADH

Alcohol dehydrogenase

ALD

Alcoholic liver damage

ALDH

Acetaldehyde dehydrogenase

AST

Aspartate transaminase

ALT

Alanine transaminase

CYP2E1

Cytochrome P450 2E1

PP

Purple potato

PPAs

Purple potato anthocyanins

GSH

Glutathione

MDA

Malondialdehyde

SOD

Superoxide dismutase

TCH

Total cholesterol

TG

Triglyceride

Notes

Acknowledgments

This work was supported by the High-end Foreign Experts Recruitment Programme of State Administration of Foreign Experts Affairs (GDW20146100228), the Ministry of Education and State Administration of Foreign Experts Affair “Overseas Teacher” Project (MS2011XBNL057), the Key Construction Program of International Cooperation Base in S&T, Shaanxi Province, China (2015SD0018), and Shannxi Provincial Science and Technology Coordinating innovative engineering project (2013ZDGC-04).

We thank Prof. Dr. Brain J. Sutton, King’s College London, for language corrections to the manuscript.

Compliance with ethical standards

Conflict of interest

The authors declare no competing financial interest.

Supplementary material

11418_2015_935_MOESM1_ESM.doc (626 kb)
Supplementary material 1 (DOC 626 kb)

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Copyright information

© The Japanese Society of Pharmacognosy and Springer Japan 2015

Authors and Affiliations

  1. 1.College of Veterinary MedicineNorthwest A&F University (North Campus)YanglingChina
  2. 2.School of Biological Science and EngineeringShaanxi University of TechnologyHanzhongChina

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