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Journal of Natural Medicines

, Volume 69, Issue 4, pp 589–594 | Cite as

Phenolic compounds from the bark of Oroxylum indicum activate the Ngn2 promoter

  • Rolly G. Fuentes
  • Midori A. AraiEmail author
  • Samir K. Sadhu
  • Firoj Ahmed
  • Masami IshibashiEmail author
Note

Abstract

A reporter gene assay that detects neurogenin 2 (Ngn2) promoter activity was utilized to identify compounds that induce neuronal differentiation. Ngn2 is a basic helix-loop-helix transcription factor that activates transcription of pro-neural genes. Using this assay system and an activity-guided approach, seven phenolic compounds were isolated from the methanol extract of Oroxylum indicum: 1 oroxylin A, 2 chrysin, 3 hispidulin, 4 baicalein, 5 apigenin, 6 baicalin, and 7 isoverbascoside. Compounds 1 and 2 induced an estimated 2.7-fold increase in Ngn2 promoter activity, whereas 3 increased the activity by 2.5-fold. Furthermore, 1 and 2 enhanced neuronal differentiation of C17.2 cells, which are multipotent stem cells.

Graphical Abstract

Keywords

bHLH Flavonoids Neurite 

Notes

Acknowledgments

We thank Prof. Dr. R. Kageyama for the Ngn2 plasmid. This study was supported by a Grant-in-Aid for Scientific Research from the Japan Society for the Promotion of Science (JSPS).

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Copyright information

© The Japanese Society of Pharmacognosy and Springer Japan 2015

Authors and Affiliations

  1. 1.Graduate School of Pharmaceutical SciencesChiba UniversityChibaJapan
  2. 2.Pharmacy Discipline, Life Science SchoolKhulna UniversityKhulnaBangladesh
  3. 3.Department of Pharmaceutical ChemistryUniversity of DhakaDhakaBangladesh
  4. 4.University of the Philippines Visayas Tacloban CollegeTacloban CityPhilippines

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