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Antidepressant-like activity of Tagetes lucida Cav. is mediated by 5-HT1A and 5-HT2A receptors

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Abstract

It has been demonstrated that the aqueous extract of Tagetes lucida Cav. shows an antidepressant-like effect on the forced swimming test (FST) in rats. The aim of this study was to analyze the participation of the serotoninergic system in the antidepressant-like effect of the aqueous extract of T. lucida. Different doses of the extract of T. lucida were administered at 72, 48, 24, 18 and 1 h before FST. The animals were pretreated with a 5-HT1A receptor antagonist (WAY-100635, 0.5 mg/kg), a 5-HT2A receptor antagonist (ketanserin, 5 mg/kg), a β-noradrenergic receptor antagonist (propranolol, 200 mg/kg), and with a α2-noradrenergic receptor antagonist (yohimbine, 1 mg/kg) alone or combined with the extract and pretreated with a serotonin synthesis inhibitor (PCPA) before treatment with 8-OH-DPAT + the extract of T. lucida. In addition, suboptimal doses of the 5-HT1A agonist (8-OH-DPAT) + non-effective dose of extract was analyzed in the FST. To determine the presence of flavonoids, the aqueous extract of T. lucida (20 µl, 4 mg/ml) was injected in HPLC; however, a quercetin concentration of 7.72 mg/g of extract weight was detected. A suboptimal dose of 8-OH-DPAT + extract of T. lucida decreased immobility and increased swimming and climbing. An antidepressant-like effect with the aqueous extract of T. lucida at doses of 100 and 200 mg/kg was observed on the FST with decreased immobility behavior and increased swimming; however, this effect was blocked by WAY-100635, ketanserin and PCPA but not by yohimbine and propranolol, suggesting that the extract of T. lucida could be modulating the release/reuptake of serotonin.

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Acknowledgments

The present study was supported by a grant from CONACyT (212823) in the form of a Fellowship awarded to Gabriela Guadarrama Cruz as part of her Doctoral research in Experimental Biology. We thank Mtra. Tania Banderas for the HPLC analysis of Tagetes lucida Cav.

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Authors and Affiliations

  1. Laboratorio de Farmacología Conductual, Dpto. Biologia de la Reproducción, D.C.B.S., Universidad Autonoma Metropolitana Iztapalapa, 09340, México, D.F., México

    H. Bonilla-Jaime

  2. Posgrado en Biologia Experimental, D.C.B.S., Universidad Autonoma Metropolitana Iztapalapa, 09340, México, D.F., México

    G. Guadarrama-Cruz & O. Limón-Morales

  3. Laboratorio de Farmacologia, D.C.B.S., Universidad Autonoma Metropolitana Iztapalapa, 09340, México, D.F., México

    F. J. Alarcon-Aguilar

  4. Colegio de Ciencia y Tecnología, Universidad Autónoma de la Ciudad de México-San Lorenzo Tezonco, Av. Prolongación San Isidro 151, Col. San Lorenzo Tezonco, Deleg, Iztapalapa, 09790, México, D.F., México

    G. Vazquez-Palacios

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  1. H. Bonilla-Jaime
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  5. G. Vazquez-Palacios
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Correspondence to H. Bonilla-Jaime.

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Bonilla-Jaime, H., Guadarrama-Cruz, G., Alarcon-Aguilar, F.J. et al. Antidepressant-like activity of Tagetes lucida Cav. is mediated by 5-HT1A and 5-HT2A receptors. J Nat Med 69, 463–470 (2015). https://doi.org/10.1007/s11418-015-0909-5

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  • DOI: https://doi.org/10.1007/s11418-015-0909-5

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