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Intestinal bacterial metabolism and anti-complement activities of three major components of the seeds of Entada phaseoloides

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Abstract

The present study aimed to investigate the metabolism of Entadae Semen by human fecal bacteria to clarify the relationship between its pharmacological activities and intestinal metabolism. Three major components (phaseoloidin, entadamide A-β-d-glucopyranoside and entadamide A) were isolated and identified from Entadae Semen and then incubated with human fecal microflora in vitro to investigate the metabolic processes. The metabolites were analyzed with high-performance liquid chromatography (HPLC). The anti-complement activities of the three components and their metabolites produced by human fecal microflora were evaluated in vitro using a hemolysis assay. Phaseoloidin and entadamide A-β-d-glucopyranoside were metabolized into their respective aglycones during the incubation process, which enhanced their anti-complement effects. These results indicated that the presence of intestinal bacteria likely plays an important role and that the pharmacological effects of Entadae Semen may be dependent on intestinal bacterial metabolism.

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Acknowledgments

This work was financially supported by the National Project in Significant Creation of New Drugs (2012ZX09103201-038) and the National Natural Science Foundation of China (No. 81173551 and No. 81274062).

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Authors and Affiliations

  1. School of Pharmacy, Shanghai Jiao Tong University, No.800 Dongchuan Road, Minhang District, Shanghai, 200240, People’s Republic of China

    Shihua Xing, Mengyue Wang, Ying Peng, Yuqiong Dong & Xiaobo Li

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  1. Shihua Xing
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  2. Mengyue Wang
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  3. Ying Peng
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  4. Yuqiong Dong
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  5. Xiaobo Li
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Correspondence to Xiaobo Li.

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Xing, S., Wang, M., Peng, Y. et al. Intestinal bacterial metabolism and anti-complement activities of three major components of the seeds of Entada phaseoloides . J Nat Med 69, 171–177 (2015). https://doi.org/10.1007/s11418-014-0874-4

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  • DOI: https://doi.org/10.1007/s11418-014-0874-4

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