l-Theanine is a unique non-protein-forming amino acid present in tea [Camellia sinensis (L.) O. Kuntze]. In the present work, we evaluated the healing effect of l-theanine on NSAID (indomethacin)-induced gastric ulcer. Histology of the stomach tissues revealed maximum ulceration on the third day after indomethacin administration (18 mg/kg, single dose p.o.) which was accompanied by increased lipid peroxidation; protein carbonylation; Th1 cytokine synthesis, and depletion of thiol, mucin, prostaglandin (PG) E, Th2 cytokine synthesis; and total antioxidant status in mice. l-Theanine healed gastric ulcer at a dose of 10 mg/kg b.w. but aggravated the ulcerated condition at a higher dose of 40 mg/kg b.w. At 10 mg/kg b.w., l-theanine significantly alleviated the adverse oxidative effect of indomethacin through enhanced synthesis of PGE2 by modulation of cyclo-oxygenase-1 and 2 [COX-1 and COX-2] expression, Th1/Th2 cytokine balance, and restoration of cellular antioxidant status at the gastric ulcer margin. The present study revealed for the first time the dose-dependent biphasic effect of a natural neuroprotective agent, l-theanine, on gastric ulcer disease.
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S.C. is grateful to Tea Board, Govt. of India, for providing a research fellowship. The authors would like to thank Mr. Narendra Kumar, SSO (Biochemistry), DTR&DC, Tea Board, Kurseong (GOI) for providing Darjeeling tea samples for l-theanine analysis and for his unconditional help throughout the work. This work was supported by funds from Tea Board, India (GOI).
S. Chatterjee and A. Chatterjee contributed equally as first author.
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Chatterjee, S., Chatterjee, A., Roy, S. et al. l-Theanine healed NSAID-induced gastric ulcer by modulating pro/antioxidant balance in gastric ulcer margin. J Nat Med 68, 699–708 (2014). https://doi.org/10.1007/s11418-014-0852-x
- Gastric ulcer