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Inhibitory effect of selected medicinal plants on the release of pro-inflammatory cytokines in lipopolysaccharide-stimulated human peripheral blood mononuclear cells

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Abstract

The inhibitory activities of the methanol extracts from 20 selected medicinal plants on the release of pro-inflammatory cytokines in human peripheral blood mononuclear cells (PBMCs) were evaluated. The major compound from the most active plant extract was also investigated. The inhibitory effect of the methanol extracts on the release of pro-inflammatory cytokines was tested by incubating PBMCs with the sample and then stimulating by lipopolysaccharide at 0.1 μg/ml. The level of cytokines was determined using enzyme-linked immunosorbent assay. Among the extracts tested, Andrographis paniculata extract demonstrated the strongest inhibition of interleukin (IL)-1β, IL-1α, and IL-6 release, with IC50 values of 1.54, 1.06, and 0.74 μg/ml, respectively. The IC50 value of A. paniculata extract was significantly higher than that of andrographolide on IL-1α, IL-1β, and IL-6 (p < 0.001) release. The IC50 values of andrographolide for IL-1α, IL-1β, and IL-6 were significantly higher (p < 0.001) than that of dexamethasone. Cymbopogon citratus and Zingiber officinale strongly inhibited the release of IL-1β, with IC50 values of 3.22 and 3.17 μg/ml, respectively. To our knowledge, this is the first report that A. paniculata extract and its major compound andrographolide strongly inhibited the release of IL-1α, whereas previous studies only showed their inhibitory effect on the release of another IL-1 family member, IL-1β. The results show that these extracts and this compound have potential effects as anti-inflammatory agents by inhibiting the release of pro-inflammatory cytokines.

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References

  1. Kumar V, Abbas AK, Fausto N, Mitchell R (2007) Robbins basic pathology, 8th edn. Elsevier Saunders, Philadelphia

    Google Scholar 

  2. Shailasree S, Ruma K, Kini KR, Niranjana SR, Prakash HS (2012) Potential anti-inflammatory bioactives from medicinal plants of Western Ghats, India. Pharmacogn Commun 2(2):2–12

    Article  CAS  Google Scholar 

  3. Aderem A, Ulevitch RJ (2000) Toll-like receptors in the induction of the innate immune response. Nature 406(6797):782–787

    Article  CAS  PubMed  Google Scholar 

  4. Saad B, Abouatta BS, Basha W, Hmade A, Kmail A, Khasib S, Said O (2011) Hypericum triquetrifolium-derived factors downregulate the production levels of LPS-induced nitric oxide and tumor necrosis factor-α in THP-1 cells. Evid Based Complement Alternat Med 2011:1–7

    Article  Google Scholar 

  5. Nantel F, Denis D, Gordon R, Northey A, Cirino M, Metters KM, Chan CC (1999) Distribution and regulation of cyclooxygenase-2 in carrageenan-induced inflammation. Br J Pharmacol 128(4):853–859

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  6. Feghali CA, Wright TM (1997) Cytokines in acute and chronic inflammation. Front Biosci 2:12–26

    Google Scholar 

  7. Beutler B, Cerami A (1989) The biology of cachectin/TNF–a primary mediator of the host response. Annu Rev Immunol 7:625–655

    Article  CAS  PubMed  Google Scholar 

  8. Delgado AV, McManus AT, Chambers JP (2003) Production of tumor necrosis factor-alpha, interleukin-1beta, interleukin-2, and interleukin-6 by rat leukocyte subpopulations after exposure to substance P. Neuropeptides 37(6):355–361

    Article  CAS  PubMed  Google Scholar 

  9. Tayal V, Kalra BS (2008) Cytokines and anti-cytokines as therapeutics: an update. Eur J Pharmacol 579:1–12

    Article  CAS  PubMed  Google Scholar 

  10. Metsios GS, Stavropoulos-Kalinoglou A, Sandoo A, van Zanten JJ, Toms TE, John H, Kitas GD (2010) Vascular function and inflammation in rheumatoid arthritis: the role of physical activity. Open Cardiovasc Med J 4(1):89–96

    Article  PubMed Central  PubMed  Google Scholar 

  11. Mantovani A, Garlanda C, Allavena P (2010) Molecular pathways and targets in cancer-related inflammation. Ann Med 42(3):161–170

    Article  CAS  PubMed  Google Scholar 

  12. Hotamisligil GS (2010) Endoplasmic reticulum stress and the inflammatory basis of metabolic disease. Cell 140(6):900–917

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  13. Frostegard J (2010) Rheumatic diseases: insights into inflammation and atherosclerosis. Arterioscler Thromb Vasc Biol 30(5):892–893

    Article  PubMed  Google Scholar 

  14. Mathieu P, Lemieux I, Despres JP (2010) Obesity, inflammation, and cardiovascular risk. Clin Pharmacol Ther 87(4):407–416

    Article  CAS  PubMed  Google Scholar 

  15. Batlouni M (2010) Nonsteroidal anti-inflammatory drugs: cardiovascular, cerebrovascular and renal effects. Arq Bras Cardiol 94(4):522–563

    Article  CAS  Google Scholar 

  16. World Medical Association (2008) Declaration of Helsinki: ethical principles for medical research involving human subjects (http://www.wma.net/en/30publications/10policies/b3/index.html)

  17. Wangchuk P, Keller PA, Pyne SG, Taweechotipatr M (2013) Inhibition of TNF-α production in LPS-activated THP-1 monocytic cells by the crude extracts of seven Bhutanese medicinal plants. J Ethnopharmacol 148(3):1013–1017

    Article  CAS  PubMed  Google Scholar 

  18. Lu Y, Liu Y, Fukuda K, Nakamura Y, Kumagai N, Nishida, T (2006) Inhibition by triptolide of chemokine, proinflammatory cytokine, and adhesion molecule expression induced by lipopolysaccharide in corneal fibroblasts. Invest Ophthalmol Vis Sci 47(9):3796–3800

    Article  PubMed  Google Scholar 

  19. Arbabi S, Rosengart MR, Garcia I, Jelacic S, Maier RV (1999) Priming interleukin 8 production: role of platelet-activating factor and p38. Arch Surg 134:1348–1353

    Article  CAS  PubMed  Google Scholar 

  20. Baggiolini M, Clark-Lewis I (1992) Interleukin-8, a chemotactic and inflammatory cytokine. FEBS 307(1):97–101

    Article  CAS  Google Scholar 

  21. Wysk M, Yang DD, Lu HT, Flavell RA, Davis RJ (1999) Requirement of mitogen-activated protein kinase kinase 3 (MKK3) for tumor necrosis factor-induced cytokine expression. Proc Natl Acad Sci USA 96:3763–3768

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  22. Francisco V, Figuerinha A, Neves BM, Rodriguez CG, Lopes MC, Cruz MT, Batista MT (2011) Cymbopogon citratus as source of new and safe anti-inflammatory drugs: bio-guided assay using lipopolysaccharide-stimulated macrophages. J Ethnopharmacol 133:818–827

    Article  CAS  PubMed  Google Scholar 

  23. Lee HJ, Jeong HS, Kim DJ, Noh YH, Yuk DY, Hong JT (2007) Inhibitory effect of citral on NO production by suppression of iNOS expression and NF-κB activation in RAW 264.7 cells. Arch Pharm Res 31:342–349

    Article  Google Scholar 

  24. Jung HW, Yoon CH, Park KM, Han HS, Park YK (2009) Hexane fraction of Zingiberis Rhizoma Crudus extract inhibits the production of nitric oxide and pro-inflammatory cytokines in LPS-stimulated BV2 microglial cells via the NF-κB pathway. Food Chem Toxicol 47:1190–1197

    Article  CAS  PubMed  Google Scholar 

  25. Kim HW, Murakami A, Abe M, Ozawa Y, Morimitsu Y, Williams MV, Ohigashi H (2005) Suppressive effects of mioga ginger and ginger constituents on reactive oxygen and nitrogen species generation, and the expression of inducible pro-inflammatory genes in macrophages. Antioxid Redox Signal 7:1621–1629

    Article  CAS  PubMed  Google Scholar 

  26. Phan PV, Sohrabi A, Polotsky A, Hungerford DS, Lindmark L, Frondoza CG (2005) Ginger extract components suppress induction of chemokine expression in human synoviocytes. J Altern Complement Med 11:149–154

    Article  PubMed  Google Scholar 

  27. Aggarwal BB, Prasad S, Reuter S, Kannapan R, Yadev VR, Park B, Kim JH, Gupta SC, Phromnoi K, Sundaram C, Prasad S, Chaturvedi MM, Sung B (2011) Identification of novel anti-inflammatory agents from ayurvedic medicine for prevention of chronic diseases: “reverse pharmacology” and “bedside to bench” approach. Curr Drug Targets 12(11):1595–1653

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  28. Sheeja K, Kuttan G (2010) Andrographis paniculata downregulates pro-inflammatory cytokine production and augments cell mediated immune response in metastatic tumor-bearing mice. Asian Pac J Cancer Prev 11:723–729

    CAS  PubMed  Google Scholar 

  29. Bao Z, Guan S, Cheng C, Wu S, Wong SH, Kemeny DM, Leung BP, Wong WS (2009) A novel antiinflammatory role for andrographolide in asthma via inhibition of the nuclear factor-kB pathway. Am J Respir Crit Care Med 179:657–665

    Article  CAS  PubMed  Google Scholar 

  30. Huang SS, Chiu CS, Chen HJ, Hou WC, Sheu MJ, Lin YC, Shie PH, Huang GJ (2011) Antinociceptive activities and the mechanisms of anti-inflammation of Asiatic acid in mice. Evid Based Complement Alternat Med 2011:1–10

    Google Scholar 

  31. Zhu T (2013) Andrographolide protects against LPS-induced acute lung injury by inactivation of NF-κB. PLoS One 8(2):1–12

    Google Scholar 

  32. Shen T (2013) AP-1/IRF-3 targeted anti-inflammatory activity of andrographolide isolated from Andrographis paniculata. Evid Based Complement Alternat Med 2013:1–16

    Google Scholar 

  33. Sims JE, Smith DE (2010) The IL-1 family: regulators of immunity. Nat Rev Immunol 10(2):89–102

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

This study was supported by the grant from Science Fund, Ministry of Agriculture Malaysia, No. 05-01-02-SF1022.

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Correspondence to Endang Kumolosasi.

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Salim, E., Kumolosasi, E. & Jantan, I. Inhibitory effect of selected medicinal plants on the release of pro-inflammatory cytokines in lipopolysaccharide-stimulated human peripheral blood mononuclear cells. J Nat Med 68, 647–653 (2014). https://doi.org/10.1007/s11418-014-0841-0

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  • DOI: https://doi.org/10.1007/s11418-014-0841-0

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