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Wound healing activities of standardized pomegranate rind extract and its major antioxidant ellagic acid in rat dermal wounds

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Abstract

The in vivo wound healing potential of a standardized pomegranate rind extract (SPRE) and its major antioxidant constituent, ellagic acid (EA, 13 %, w/w), were investigated in three rat dermal wound models. It was found that both SPRE (5 and 2.5 %) and its equivalent amount of EA (0.65 and 0.325 %) increased the tensile strength of the incision wound by a maximum of 35.43 and 31.82 %, respectively. SPRE at 5 and 2.5 % accelerated wound contraction of the excision wound and the burn wound, while EA was effective only at 0.65 % in these two wound models. Further assays revealed that SPRE enhanced the synthesis of collagen by a maximum of 21.83 mg/g and inhibited neutrophil infiltration dose-dependently, while EA was not effective in increasing collagen accumulation and its inhibitory effect on neutrophil infiltration was milder. These results indicated that SPRE is a promising phytopharmaceutical effective in facilitating the healing of wounds and is superior to its marker compound EA.

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Acknowledgment

This research is supported by a grant from Prince of Songkla University (Grant No. PHA550382S). Thanks to School of Pharmaceutical Science and Yunnan Key Laboratory of Pharmacology for Natural Products, Kunming Medical University for some facilities and technical support. Thanks to Dr. Brian Hodgson for assistance with the English.

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Authors and Affiliations

  1. Department of Clinical Pharmacy, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Hat Yai, Songkhla, 90110, Thailand

    Jiao Mo & Wantana Reanmongkol

  2. Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Hat Yai, Songkhla, 90110, Thailand

    Pharkphoom Panichayupakaranant

  3. Department of Pharmaceutical Technology, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Hat Yai, Songkhla, 90110, Thailand

    Nattha Kaewnopparat

  4. Department of Pathology, Faculty of Medicine, Prince of Songkla University, Hat Yai, Songkhla, 90110, Thailand

    Anupong Nitiruangjaras

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  1. Jiao Mo
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Correspondence to Wantana Reanmongkol.

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Mo, J., Panichayupakaranant, P., Kaewnopparat, N. et al. Wound healing activities of standardized pomegranate rind extract and its major antioxidant ellagic acid in rat dermal wounds. J Nat Med 68, 377–386 (2014). https://doi.org/10.1007/s11418-013-0813-9

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  • DOI: https://doi.org/10.1007/s11418-013-0813-9

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