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An approach based on antioxidant fingerprint–efficacy relationship and TLC bioautography assay to quality evaluation of Rubia cordifolia from various sources

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Abstract

In this work, an approach based on antioxidant fingerprint–efficacy relationships and TLC bioautography assay was developed for quality evaluation of traditional Chinese medicine (TCM). First, chemical fingerprints of 20 batches of different sources of Rubia cordifolia were established by HPLC. The antioxidant activities of these samples were evaluated by 1,1-diphenyl-2-picrylhydrazyl radical scavenging assay. Then, five active components from fingerprint peaks were determined by both multiple correlation analysis and TLC bioautography assay. Similarity analysis and hierarchical clustering analysis (HCA) based on these active peaks were chosen to evaluate the quality of R. cordifolia from different sources. The samples with similarities below 0.9 had poorer antioxidant activities and those having stronger activities fell into the same cluster in HCA. Finally, five batches of commercial samples were processed in the same way to verify the feasibility of this method. The results suggested that chemical fingerprinting combined with similarity analysis based on fingerprint–efficacy relationship and TLC bioautography could evaluate the antioxidant activity of R. cordifolia from various sources. The established technique would also provide an easy method of evaluating the quality of other TCMs.

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References

  1. Drug Administration Bureau of China (2002) Requirements for studying fingerprint of traditional Chinese medicine injection. Drug Administration Bureau of China, Beijing

    Google Scholar 

  2. World Health Organization (1991) Guidelines for the assessment of herbal medicines, vol 28. WHO, Munich, p 6

    Google Scholar 

  3. Welsh WJ, Lin WK, Tersigni SH, Collantes E, Duta R, Carey MS (1996) Pharmaceutical fingerprinting: evaluation of neural networks and chemometric techniques for distinguishing among same-product manufacturers. Anal Chem 68:3473–3482

    Article  CAS  PubMed  Google Scholar 

  4. Collantes ER, Duta R, Welsh WJ, Zielinski WL, Brower J (1997) Preprocessing of HPLC trace impurity patterns by wavelet packets for pharmaceutical fingerprinting using artificial neural networks. Anal Chem 69:1392–1397

    Article  CAS  PubMed  Google Scholar 

  5. Aksenova TI, Tetko G IV, Ivakhnenko A, Villa AEP, Welsh WJ, Zielinski WL (1999) Pharmaceutical fingerprinting in phase space. 1. Construction of phase fingerprints. Anal Chem 71:2423–2430

    Article  CAS  PubMed  Google Scholar 

  6. Li JF, Hibbert DB (2005) Comparison of spectra using a Bayesian approach. An argument using oil spills as an example. Anal Chem 77:639–644

    Article  CAS  PubMed  Google Scholar 

  7. Gong F, Liang YZ, Xie PS, Chau FT (2003) Information theory applied to chromatographic fingerprint of herbal medicine for quality control. J Chromatogr A 1002:25–40

    Article  CAS  PubMed  Google Scholar 

  8. The Pharmacopoeia Committee of China (2010) Chinese Pharmacopoeia. Chemical industry publishing house, Beijing, pp 218–219

    Google Scholar 

  9. Liu R, Lu YB, Wu TX, Pan YJ (2008) Simultaneous isolation and purification of Mollugin and two anthraquinones from Rubia cordifolia by HSCCC. Chromatographia 68:95–99

    Article  CAS  Google Scholar 

  10. Tripathi YB, Sharma M (1999) The interaction of Rubia cordifolia with iron redox status: a mechanistic aspect in free radical reactions. Phytomedicine 6:51–57

    Article  CAS  PubMed  Google Scholar 

  11. Ma WY, Lu YB, Dai XJ, Liu R, Hu RL, Pan YJ (2009) Determination of anti-tumor constitute mollugin from traditional Chinese medicine Rubia cordifolia: comparative study of classical and microwave extraction techniques. Sep Sci Tech 44:995–1006

    Article  CAS  Google Scholar 

  12. Hsu B, Coupar IM, Ng K (2006) Antioxidant activity of hot water extract from the fruit of the Doum palm, Hyphaene thebaica. Food Chem 98:317–328

    Article  CAS  Google Scholar 

  13. Lawson KR, Lawson J (2012) LICSS—a chemical spreadsheet in Microsoft Excel. J Cheminform 4:3

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  14. Singh RP, Chidambara Murthy KN, Jayaprakasha GK (2002) Studies on the antioxidant activity of pomegranate (Punica granatum) peel and seed extracts using in vitro models. J Agric Food Chem 50:81–86

    Article  CAS  PubMed  Google Scholar 

  15. Gumus E, Kursun O, Sertbas A, Ustek D (2012) Application of canonical correlation analysis for identifying viral integration preferences. Bioinformatics 28:651–655

    Article  CAS  PubMed  Google Scholar 

  16. Kannel PR, Lee S, Kanel SR, Khan SP (2007) Chemometric application in classification and assessment of monitoring locations of an urban river system. Anal Chim Acta 582:390–399

    Article  CAS  PubMed  Google Scholar 

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Acknowledgments

This research work was financially supported by Harbin Science and Technology Bureau (2012RFQXS048).

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Authors and Affiliations

  1. Department of Pharmaceutical Engineering, Heilongjiang University, Harbin, 150080, China

    Xu-Jie Zhang, Li-Juan Liu & Ting-Ting Song

  2. Key Laboratory of Chemical Engineering Process and Technology for High-efficiency Conversion, College of Heilongjiang Province, Harbin, 150080, China

    Li-Juan Liu

  3. Center for Disease Control and Prevention, Shangzhi, 150600, China

    Yan-Qiu Wang

  4. College of Pharmacy, Jilin University, Changchun, 130021, China

    Xiao-hong Yang

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  1. Xu-Jie Zhang
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  2. Li-Juan Liu
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  3. Ting-Ting Song
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  4. Yan-Qiu Wang
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  5. Xiao-hong Yang
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Correspondence to Li-Juan Liu.

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Zhang, XJ., Liu, LJ., Song, TT. et al. An approach based on antioxidant fingerprint–efficacy relationship and TLC bioautography assay to quality evaluation of Rubia cordifolia from various sources. J Nat Med 68, 448–454 (2014). https://doi.org/10.1007/s11418-013-0812-x

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  • DOI: https://doi.org/10.1007/s11418-013-0812-x

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