Abstract
A polyhydroxyoctane, 6-methylheptane-1,2,3,4,5-pentaol (MHP), was first isolated from mycelia of the Thai edible mushroom Lentinus polychrous. MHP was evaluated for its cytotoxic and immunomodulatory effects in vitro. MHP was slightly cytotoxic to murine splenocytes but not to RAW264.7 cells or peripheral blood mononuclear cells. MHP decreased nitric oxide and intracellular O2 − production from lipopolysaccharide- and phorbol-12-myristate-13-acetate-activated RAW264.7 cells at levels of 78.98 ± 4.72 and 78.48 ± 2.41 % of controls, respectively. The mRNA expression of pro-inflammatory mediators, including iNOS, TNF-α, IL-1β, IL-6, COX-1 and COX-2, were significantly suppressed by MHP. In addition, MHP significantly increased the proliferation of phytohemagglutinin and pokeweed mitogen-induced splenocytes. These results indicate that MHP is able to modulate inflammatory responses and the proliferation of both T- and B-lymphocyte cells, suggesting that MHP may be a good natural immunomodulator.
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Acknowledgments
The authors would like to thank the Office of the Higher Education Commission, Thailand for a scholarship under the program Strategic Scholarships for Frontier Research Network for the PhD Program. This study was also financially supported by the Center of Research and Development of Herbal Health Products, Khon Kaen University. In addition, we would like to thank the Laboratory of Pharmacognosy, School of Pharmaceutical Sciences, University of Shizuoka, Shizuoka, Japan for research funds and instrument support.
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Fangkrathok, N., Junlatat, J., Umehara, K. et al. Cytotoxic and immunomodulatory effects of polyhydroxyoctane isolated from Lentinus polychrous mycelia. J Nat Med 68, 302–309 (2014). https://doi.org/10.1007/s11418-013-0797-5
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DOI: https://doi.org/10.1007/s11418-013-0797-5