Journal of Natural Medicines

, Volume 67, Issue 4, pp 850–855

Astragaloside content in the periderm, cortex, and xylem of Astragalus membranaceus root

  • Ha-Jeong Kwon
  • Jeehyun Hwang
  • Sun-Kyoung Lee
  • Yong-Duk Park
Note

Abstract

Astragalosides are among the most predominant of the bioactive compounds in the root of Astragalus membranaceus and are differentially concentrated depending on the anatomical part of the root in question. The aim of this study was to analyse astragaloside contents in the periderm, cortex, and xylem of A. membranaceus root, and to compare the contents between peeled and unpeeled roots. Total astragalosides in the periderm were about 8-fold more concentrated than in the cortex, and 28-fold more concentrated than in the xylem. The dry weight percentages of total astragalosides in primary roots were 43.5 % in the periderm, 47.2 % in the cortex, and 9.30 % in the xylem. Furthermore, unpeeled main (primary) roots were enriched in astragalosides by 1.46-fold compared with peeled main roots, whereas unpeeled lateral roots were enriched by 2.33-fold compared with peeled lateral roots. In conclusion, the periderm is the most astragaloside-rich part of the root of A. membranaceus. Therefore, it is necessary to preserve the periderm in order to supply astragaloside-rich roots for use as health food supplements.

Keywords

Astragalus membranaceus Astragaloside Periderm Peeled root Unpeeled root 

Supplementary material

11418_2013_741_MOESM1_ESM.docx (13.2 mb)
Supplementary material 1 (DOCX 13565 kb)

References

  1. 1.
    KFDA (2007) Korean pharmacopoeia, 9th edn. Shinil Books Co Ltd, SeoulGoogle Scholar
  2. 2.
    Zheng KY, Choi RC, Cheung AW, Guo AJ, Bi CW, Zhu KY, Fu Q, Du Y, Zhang WL, Zhan JY, Duan R, Lau DT, Dong TT, Tsim KW (2011) Flavonoids from Radix Astragali induce the expression of erythropoietin in cultured cells: a signaling mediated via the accumulation of hypoxia-inducible factor-1α. J Agric Food Chem 59:1697–1704PubMedCrossRefGoogle Scholar
  3. 3.
    Wang N, Zhang D, Mao X, Zou F, Jin H, Ouyang J (2009) Astragalus polysaccharides decreased the expression of PTP1B through relieving ER stress induced activation of ATF6 in a rat model of type 2 diabetes. Mol Cell Endocrinol 307:89–98PubMedCrossRefGoogle Scholar
  4. 4.
    Zou F, Mao XQ, Wang N, Liu J, Ou-Yang JP (2009) Astragalus polysaccharides alleviates glucose toxicity and restores glucose homeostasis in diabetic states via activation of AMPK. Acta Pharmacol Sin 30:1607–1615PubMedCrossRefGoogle Scholar
  5. 5.
    Liu M, Wu K, Mao X, Wu Y, Ouyang J (2010) Astragalus polysaccharide improves insulin sensitivity in KKAy mice: regulation of PKB/GLUT4 signaling in skeletal muscle. J Ethnopharmacol 127:32–37PubMedCrossRefGoogle Scholar
  6. 6.
    Tang D, He B, Zheng ZG, Wang RS, Gu F, Duan TT, Cheng HQ, Zhu Q (2011) Inhibitory effects of two major isoflavonoids in Radix Astragali on high glucose-induced mesangial cells proliferation and AGEs-induced endothelial cells apoptosis. Planta Med 77:729–732PubMedCrossRefGoogle Scholar
  7. 7.
    Li RJ, Qiu SD, Chen HX, Tian H, Wang HX (2007) The immunotherapeutic effects of Astragalus polysaccharide in type 1 diabetic mice. Biol Pharm Bull 30:470–476PubMedCrossRefGoogle Scholar
  8. 8.
    Ryu M, Kim EH, Chun M, Kang S, Shim B, Yu YB, Jeong G, Lee JS (2008) Astragali Radix elicits anti-inflammation via activation of MKP-1, concomitant with attenuation of p38 and Erk. J Ethnopharmacol 115:184–193PubMedCrossRefGoogle Scholar
  9. 9.
    Gao XH, Xu XX, Pan R, Li Y, Luo YB, Xia YF, Murata K, Matsuda H, Dai Y (2009) Saponin fraction from Astragalus membranaceus roots protects mice against polymicrobial sepsis induced by cecal ligation and puncture by inhibiting inflammation and upregulating protein C pathway. J Nat Med 63:421–429PubMedCrossRefGoogle Scholar
  10. 10.
    Meng L, Van Putten V, Qu L, Nemenoff RA, Shang MY, Cai SQ, Li X (2010) Altered expression of genes profiles modulated by a combination of Astragali Radix and Angelicae Sinensis Radix in obstructed rat kidney. Planta Med 76:1431–1438PubMedCrossRefGoogle Scholar
  11. 11.
    Zhang Y, Hu G, Lin HC, Hong SJ, Deng YH, Tang JY, Seto SW, Kwan YW, Waye MM, Wang YT, Lee SM (2009) Radix Astragali extract promotes angiogenesis involving vascular endothelial growth factor receptor-related phosphatidylinositol 3-kinase/Akt-dependent pathway in human endothelial cells. Phytother Res 23:1205–1213PubMedCrossRefGoogle Scholar
  12. 12.
    Bedir E, Pugh N, Calis I, Pasco DS, Khan IA (2000) Immunostimulatory effects of cycloartane-type triterpene glycosides from Astragalus species. Biol Pharm Bull 23:834–837PubMedCrossRefGoogle Scholar
  13. 13.
    Gariboldi P, Pelizzoni F, Tatò M, Verotta L, el-Sebakhy N, Asaad AM, Abdallah RM, Toaima SM (1995) Cycloartane triterpene glycosides from Astragalus trigonus. Phytochemistry 40:1755–1760Google Scholar
  14. 14.
    Rios JL, Waterman PG (1997) A review of the pharmacology and toxicology of Astragalus. Phytother Res 11:411–418CrossRefGoogle Scholar
  15. 15.
    Pistelli L (2002) Secondary metabolites of genus Astragalus: structure and biological activity. In: Atta-Ur-Rahman (ed) Studies in natural products chemistry (Bioactive natural products, Part H). Elsevier, Karachi, pp 443–545Google Scholar
  16. 16.
    Tang L, Liu Y, Wang Y, Long C (2010) Phytochemical analysis of an antiviral fraction of Radix astragali using HPLC-DAD-ESI-MS/MS. J Nat Med 64:182–186PubMedCrossRefGoogle Scholar
  17. 17.
    Bian Q, Huang JH, Liang QQ, Shu B, Hou W, Xu H, Zhao YJ, Lu S, Shi Q, Wang YJ (2011) The osteogenetic effect of astragaloside IV with centrifugating pressure on the OCT-1 cells. Pharmazie 66:63–68PubMedGoogle Scholar
  18. 18.
    Luo Y, Qin Z, Hong Z, Zhang X, Ding D, Fu JH, Zhang WD, Chen J (2004) Astragaloside IV protects against ischemic brain injury in a murine model of transient focal ischemia. Neurosci Lett 363:218–223PubMedCrossRefGoogle Scholar
  19. 19.
    Zhang L, Liu Q, Lu L, Zhao X, Gao X, Wang Y (2011) Astragaloside IV stimulates angiogenesis and increases hypoxia-inducible factor-1α accumulation via phosphatidylinositol 3-kinase/Akt pathway. J Pharmacol Exp Ther 338:485–491PubMedCrossRefGoogle Scholar
  20. 20.
    Zhang N, Wang XH, Mao SL, Zhao F (2011) Astragaloside IV improves metabolic syndrome and endothelium dysfunction in fructose-fed rats. Molecules 16:3896–3907PubMedCrossRefGoogle Scholar
  21. 21.
    Xu XL, Chen XJ, Ji H, Li P, Bian YY, Yang D, Xu JD, Bian ZP, Zhang JN (2008) Astragaloside IV improved intracellular calcium handling in hypoxia-reoxygenated cardiomyocytes via the sarcoplasmic reticulum Ca-ATPase. Pharmacology 81:325–332PubMedCrossRefGoogle Scholar
  22. 22.
    Zhang W, Zhang C, Liu R, Li H, Zhang J, Mao C, Chen C (2005) Quantitative determination of astragaloside IV, a natural product with cardioprotective activity, in plasma, urine and other biological samples by HPLC coupled with tandem mass spectrometry. J Chromatogr B Analyt Technol Biomed Life Sci 822:170–177PubMedCrossRefGoogle Scholar
  23. 23.
    Yu QT, Qi LW, Li P, Yi L, Zhao J, Bi Z (2007) Determination of seventeen main flavonoids and saponins in the medicinal plant Huang-qi (Radix astragali) by HPLC-DAD-ELSD. J Sep Sci 30:1292–1299PubMedCrossRefGoogle Scholar
  24. 24.
    Wang CZ, Wu JA, McEntee E, Yuan CS (2006) Saponins composition in American ginseng leaf and berry assayed by high-performance liquid chromatography. J Agric Food Chem 54:2261–2266PubMedCrossRefGoogle Scholar
  25. 25.
    Wang Y, Pan JY, Xiao XY, Lin RC, Cheng YY (2006) Simultaneous determination of ginsenosides in Panax ginseng with different growth ages using high-performance liquid chromatography-mass spectrometry. Phytochem Anal 17:424–430PubMedCrossRefGoogle Scholar
  26. 26.
    Christensen LP, Jensen M, Kidmose U (2006) Simultaneous determination of ginsenosides and polyacetylenes in American ginseng root (Panax quinquefolium L.) by high-performance liquid chromatography. J Agric Food Chem 54:8995–9003PubMedCrossRefGoogle Scholar
  27. 27.
    Baum SF, Dubrovsky JG, Rost TL (2002) Apical organization and maturation of the cortex and vascular cylinder in Arabidopsis thaliana (Brassicaceae) roots. Am J Bot 89:908–920PubMedCrossRefGoogle Scholar
  28. 28.
    Guan J, Bi ZM, Li P (2006) Study on fingerprints of astragalosides injection by HPLC-ELSD. Zhongguo Zhong Yao Za Zhi 31:807–809PubMedGoogle Scholar
  29. 29.
    Kwon HJ, Sim HJ, Lee SI, Lee YM, Lee JH, Park YD, Hong SP (2011) Analysis of saikosaponins in Bupleuri Radix and Caihu-shugan-san using reversed-phase HPLC with pulsed amperometric detection. J Sep Sci 34:651–659PubMedCrossRefGoogle Scholar
  30. 30.
    Cataldi TRI, Nardiello D (2003) Determination of free proline and monosaccharides in wine samples by high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD). J Agric Food Chem 51:3737–3742PubMedCrossRefGoogle Scholar
  31. 31.
    Kwon HJ, Park YD (2012) Determination of astragalin and astragaloside content in Radix Astragali using high-performance liquid chromatography coupled with pulsed amperometric detection. J Chromatogr A 1232:212–217PubMedCrossRefGoogle Scholar

Copyright information

© The Japanese Society of Pharmacognosy and Springer Japan 2013

Authors and Affiliations

  • Ha-Jeong Kwon
    • 1
  • Jeehyun Hwang
    • 1
  • Sun-Kyoung Lee
    • 1
  • Yong-Duk Park
    • 1
  1. 1.Department of Preventive and Social Dentistry, Graduate SchoolKyung Hee UniversitySeoulSouth Korea

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