Effect of Cissampelos pareira root extract on isoproterenol-induced cardiac dysfunction
The aim of this study was to assess the cardioprotective effect of Cissampelos pareira root extract on isoproterenol-induced cardiac dysfunction in rats. Male albino Wistar rats were randomly divided into eight groups and received either normal saline (0.5 ml/kg, intraperitoneally), isoproterenol (5 mg/kg, intraperitoneally), C. pareira (100 and 200 mg/kg, by gavage, respectively) alone, amlodipine (9 mg/kg, by gavage) alone, C. pareira (100 and 200 mg/kg, respectively) + isoproterenol and amlodipine (9 mg/kg) + isoproterenol, once a day for 30 days, respectively. Isoproterenol-induced cardiac dysfunction was characterized by a significant (P < 0.001) increase in the heart weigh/body weight ratio, serum calcineurin, nitric oxide, lactate dehydrogenase, and thiobarbituric acid reactive substance levels, as well as a significant decrease in serum-reduced glutathione, cardiac glutathione peroxidase, glutathione reductase, and glutathione-S-transferase levels, which were significantly (P < 0.05 and P < 0.01) improved by C. pareira treatment. No significant alteration was observed in the group treated with C. pareira alone compared with the control. C. pareira treatment also restored histopathological changes observed in isoproterenol-induced rats. Amlodipine is used as standard drug in this study. Thus, these results suggest that the attenuation of isoproterenol-induced cardiac dysfunction by treatment with ethanolic root extract of C. pareira may be due to amelioration of calcineurin activity and free radical formation, and by augmentation of antioxidant enzymes activities.
KeywordsCissampelos pareira Isoproterenol Cardiac hypertrophy Oxidative stress Calcineurin
The authors are grateful to University Grants Commission, New Delhi, for providing financial assistance in the form of UGC-BSR fellowship. Thanks are also due to Ranbaxy Laboratory, Gurgaon and Torrent Pharmaceuticals Limited, Baddi, India for providing amlodipine besylate, and trifluoperazine hydrochloride, respectively.
Conflict of interest
- 11.Anonymous (1992) Wealth of India: raw materials, vol 3. Council of Scientific and Industrial Research Publication, New DelhiGoogle Scholar
- 13.Kiritikar KR, Basu BD (2000) Indian medicinal plants, vol 1. Sri Satguru Publications, DelhiGoogle Scholar
- 16.Bafna AR, Mishra SH (2005) Immunomodulatory activity of methanol extract of roots of Cissampelos pareira Linn. Ars Pharmaceutica 46:253–262Google Scholar
- 18.DeFreitas MR, Cortes SF, Thomas G, Barbosa Filho JM (1996) Modification of Ca2+-metabolism in the rabbit aorta as a mechanism of spasmolytic action of warifteine, a bisbenzylisoquinoline alkaloid isolated from the leaves of Cissampelos sympodialis Eichl. (Menispermaceae). J Pharm Pharmacol 48:332–336CrossRefGoogle Scholar
- 19.Wei-Xing Y, Ming-Xing J (2002) Effects of tetrandrine on cardiovascular electrophysiologic properties. Acta Pharmacol Sin 23:1069–1074Google Scholar
- 21.Trease GE, Evans WC (1989) Pharmacognosy. Brailliar Tiridel and Macmillian Publishers, LondonGoogle Scholar
- 27.Clairborne A (1985) Assay of catalase. In: Greenwald RA (ed) Hand book of methods of oxygen free radical research. CRC Press, Boca Raton, pp 283–284Google Scholar
- 32.Lowary OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275Google Scholar
- 33.Rana OR, Saygili E, Meyer C, Gemein C, Krüttgen A, Andrzejewski MG, Ludwig A, Schotten U, Schwinger RH, Weber C, Weis J, Mischke K, Rassaf T, Kelm M, Schauerte P (2009) Regulation of nerve growth factor in the heart: the role of the calcineurin-NFAT pathway. J Mol Cell Cardiol 46:568–578PubMedCrossRefGoogle Scholar
- 35.Hu A, Jiao X, Gao E, Koch WJ, Sharifi-Azad S, Grunwald Z, Ma XL, Sun JZ (2006) Chronic beta-adrenergic receptor stimulation induces cardiac apoptosis and aggravates myocardial ischemia/reperfusion injury by provoking inducible nitric-oxide synthase-mediated nitrative stress. J Pharmacol Exp Ther 318:469–475PubMedCrossRefGoogle Scholar