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Herbal formula HMC05 prevents human aortic smooth muscle cell migration and proliferation by inhibiting the ERK1/2 MAPK signaling cascade

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Abstract

HMC05 is a formulation derived from eight medicinal herbs, and prevents neointima formation by inhibition of the mitogen-activated protein kinase (MAPK) pathway with induction of heat shock protein 27 expression. In this study, we investigated the influence of HMC05 regulation on the MAPK/extracellular signal-regulated kinase (ERK) 1/2 signaling cascade in the inhibition of the migration and proliferation of human aortic smooth muscle cells (HASMCs). The inhibitory effects of HMC05 (25, 50, and 100 μg/ml) on tumor necrosis factor-alpha (TNF-α; 0 or 100 ng/ml)-induced HASMC migration and proliferation were investigated by wound migration and proliferation assays, Western blotting and reverse transcription-polymerase chain reaction. HMC05 completely inhibited TNF-α-induced HASMC migration and proliferation. HMC05 prevented TNF-α receptor 1-mediated phosphorylation of signal transduction molecules involved in MAPK signaling cascades such as MEK1/2, ERK1/2, Elk-1 transcription factor and p90 kDa ribosomal S6 kinase. The expression of matrix metalloproteinase, a modulator of vascular smooth muscle cell proliferation and migration, was inhibited by HMCO5 treatment, as was TNF-α-induced mRNA expression of intracellular adhesion molecule 1 and vascular cell adhesion molecule 1. HMC05 disruption of the MEK/ERK/Elk-1 and p90RSK pathways prevents HASMC migration and proliferation.

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Acknowledgments

This work was supported by the Ministry of Health & Welfare, Republic of Korea (B080031) and the Dongguk University Research Fund of 2011.

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Authors and Affiliations

  1. Department of Physiology, College of Oriental Medicine, Dongguk University, Gyeongju, 780-714, Republic of Korea

    Yun Hwan Kang, In Jun Yang & Heung Mook Shin

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  1. Yun Hwan Kang
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  2. In Jun Yang
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Correspondence to Heung Mook Shin.

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Kang, Y.H., Yang, I.J. & Shin, H.M. Herbal formula HMC05 prevents human aortic smooth muscle cell migration and proliferation by inhibiting the ERK1/2 MAPK signaling cascade. J Nat Med 66, 177–184 (2012). https://doi.org/10.1007/s11418-011-0573-3

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  • DOI: https://doi.org/10.1007/s11418-011-0573-3

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