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Qualitative and quantitative determination of ten iridoids and secoiridoids in Gentiana straminea Maxim. by LC-UV-ESI-MS

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Abstract

A simple and accurate HPLC-UV/MS method was developed for the simultaneous determination of ten iridoids and secoiridoids in the roots of Gentiana straminea Maxim. Separations were performed on a Kromasil-C18 column by gradient elution using methanol and water containing phosphoric acid. Analytes were identified by HPLC coupled with ESI-MS experiments. The chromatographic method was validated for selectivity, linearity, precision, limit of detection, limit of quantification, accuracy, and stability. The developed assay could be considered as a suitable quality control method for G. straminea and other “Qinjiao” herbs.

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Acknowledgments

This work was financially supported by Young Teacher Grant from Beijing University of Chemical Technology (QN0615) and the Fundamental Research Funds for the Central Universities (ZZ1023).

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Authors and Affiliations

  1. College of Life Science and Technology, Beijing University of Chemical Technology, No. 15 Beisanhuan East Road, Beijing, 100029, People’s Republic of China

    Shihu Wei, Changyuan Yu & Guang Chen

  2. Department of Urology, Medical School, Kochi University, Nankoku, Kochi, 783-8505, Japan

    Shihu Wei

  3. Institute of Materia Medica, Chinese Academy of Medical Sciences, No. 1 Xian Nong Tan Street, Beijing, 100050, People’s Republic of China

    Peicheng Zhang

  4. Department of Pathology and Laboratory Medicine, University of Cincinnati College of Medicine, Cincinnati, OH, 45227, USA

    Xizhi Feng

  5. Department of Anesthesiology and Critical Care Medicine, Medical School, Kochi University, Nankoku, Kochi, 783-8505, Japan

    Hiroyuki Kodama

Authors
  1. Shihu Wei
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  2. Peicheng Zhang
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  3. Xizhi Feng
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  4. Hiroyuki Kodama
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  5. Changyuan Yu
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  6. Guang Chen
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Corresponding authors

Correspondence to Changyuan Yu or Guang Chen.

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Wei, S., Zhang, P., Feng, X. et al. Qualitative and quantitative determination of ten iridoids and secoiridoids in Gentiana straminea Maxim. by LC-UV-ESI-MS. J Nat Med 66, 102–108 (2012). https://doi.org/10.1007/s11418-011-0560-8

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  • DOI: https://doi.org/10.1007/s11418-011-0560-8

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