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Protective effect of Pueraria tuberosa DC. embedded biscuit on cisplatin-induced nephrotoxicity in mice

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Abstract

Recently, the nephroprotective property of Pueraria tuberosa DC. tuber (PT) has been reported by our group. Here, PT-embedded biscuits were prepared and tested on cisplatin-induced nephrotoxicity in Swiss albino mice. The PT powder was characterized by RAPD (random amplified polymorphic DNA) to ascertain its authenticity and PT biscuits were prepared in different concentrations (1, 2, or 4 g of PT powder). These biscuits were given as diet for a total of 10 days, but on the 7th day cisplatin injection (8 mg/kg bw, i.p.) was given. On the 10th day animals were killed to collect kidneys for assessment of antioxidant status. Blood samples were collected on both the 7th and 10th days for assessment of liver and kidney functions. In mice, PT biscuit showed significant protection against cisplatin-induced nephrotoxicity, but there was a transient rise in alanine aminotransferase and aspartate aminotransferase at the dose of 4 g PT biscuit. Therefore, it is suggested that PT biscuit might be an effective food supplement for cancer patients undergoing cisplatin-chemotherapy. However, periodical liver function monitoring is required, especially when PT is used for longer periods or at higher doses.

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Acknowledgments

The authors are thankful to the Council of Scientific and Industrial Research and the Board of Research in Nuclear Sciences for financial assistance to conduct this study. The authors would also like to express their thanks to Bananas Hindu University for providing a scholarship for the associated student.

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Authors and Affiliations

  1. Department of Medicinal Chemistry, Institute of Medical Sciences, Banaras Hindu University, Varanasi, 221005, India

    Yamini B. Tripathi & Santosh Nagwani

  2. Centre of Food Science and Technology, Institute of agricultural Sciences, Banaras Hindu University, Varanasi, 221005, India

    Pooja Mishra & Alok Jha

  3. Department of Botany, Faculty of Science, Banaras Hindu University, Varanasi, 221005, India

    Shashi Pandey Rai

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  1. Yamini B. Tripathi
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  2. Santosh Nagwani
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Correspondence to Yamini B. Tripathi.

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Tripathi, Y.B., Nagwani, S., Mishra, P. et al. Protective effect of Pueraria tuberosa DC. embedded biscuit on cisplatin-induced nephrotoxicity in mice. J Nat Med 66, 109–118 (2012). https://doi.org/10.1007/s11418-011-0559-1

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  • DOI: https://doi.org/10.1007/s11418-011-0559-1

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