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Phellinus baumii ethyl acetate extract inhibits lipopolysaccharide-induced iNOS, COX-2, and proinflammatory cytokine expression in RAW264.7 cells

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Abstract

Mushrooms are valuable sources of biologically active compounds possessing anticancer, antiplatelet, and anti-inflammatory properties. Phellinus baumii is a mushroom used in folk medicine for a variety of human diseases. However, its potential anti-inflammatory effect has remained unclear. Therefore, we studied the effect of P. baumii ethyl acetate extract (PBEAE) on inflammatory mediator and proinflammatory cytokine protein and/or mRNA expression levels using the nitric oxide (NO) assay, enzyme immunoassay (EIA), western blot, and reverse transcription polymerase chain reaction (RT–PCR) in lipopolysaccharide (LPS)-stimulated macrophage like RAW264.7 cells. PBEAE markedly inhibited NO generation and prostaglandin E2 (PGE2) synthesis in a concentration-dependent pattern without any cytotoxic effect at the concentration range used. PBEAE also suppressed inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) protein expression. In addition, LPS-induced iNOS and COX-2 mRNA expression levels were dose-dependently inhibited by PBEAE pretreatment. Furthermore, PBEAE attenuated the mRNA expression levels of proinflammatory cytokines, specifically interleukin (IL)-1β, IL-6, and granulocyte macrophage colony-stimulating factor (GM-CSF), in a concentration-dependent fashion. Our study suggests that P. baumii might exhibit anti-inflammatory properties by downregulating proinflammatory mediators. Thus, further study on compounds isolated from PBEAE is warranted to investigate the associated molecular mechanisms and identify the potential therapeutic targets.

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Authors and Affiliations

  1. College of Veterinary Medicine, Kyungpook National University, Daegu, 702-701, Republic of Korea

    Taddesse Yayeh, Won Jun Oh, Seung-Choon Park, Tae-Hwan Kim & Man Hee Rhee

  2. School of Life Science and Biotechnology, Sungkyunkwan University, Suwon, 440-746, Republic of Korea

    Jae Youl Cho

  3. College of Biomedical Science and Engineering, and Regional Research Center, Inje University, Gimhae, 621-749, Republic of Korea

    Hwa-Jin Park

  4. Division of Biotechnology and Advanced Institute of Environment and Bioscience, College of Environmental and Bioresource Sciences, Chonbuk National University, Iksan, Jeonbuk, 570-752, Republic of Korea

    In-Kyoung Lee & Bong-Sik Yun

  5. College of Veterinary Medicine, Chungnam National University, Taejon, 305-764, Republic of Korea

    Sang-Keun Kim

  6. Department of Clinical Pathology, Juseong University, Chungbuk, 363-794, Republic of Korea

    Seung-Bok Hong

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  1. Taddesse Yayeh
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  2. Won Jun Oh
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Correspondence to Bong-Sik Yun or Man Hee Rhee.

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Yayeh, T., Oh, W.J., Park, SC. et al. Phellinus baumii ethyl acetate extract inhibits lipopolysaccharide-induced iNOS, COX-2, and proinflammatory cytokine expression in RAW264.7 cells. J Nat Med 66, 49–54 (2012). https://doi.org/10.1007/s11418-011-0552-8

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  • DOI: https://doi.org/10.1007/s11418-011-0552-8

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