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In vitro screening for antihyperlipidemic activities in foodstuffs by evaluating lipoprotein profiles secreted from human hepatoma cells

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Abstract

We screened the antihyperlipidemic effects of seven edible plants by evaluation of the triglyceride (TG) and cholesterol profiles secreted from HepG2 cells. We found that the water- and ethanol-extracts of Brasenia schreberi at 100 μg/ml exhibited strong inhibitory activities against TG and cholesterol secretions from HepG2 cells stimulated with sodium oleate. Real-time RT-PCR analysis demonstrated that ethanol extract of B. schreberi (BSET) attenuated the expression of the sterol regulatory element binding protein-1c and -2, fatty acid synthase and HMG CoA synthase-1 genes, which are involved in lipid synthesis in hepatocyte/hepatoma cells. Furthermore, we studied the action of BSET on adipose tissue accumulation and serum parameters in mice fed a high-fat diet (HFD). BSET suppressed mesenteric and epididymal adipose tissue accumulation and normalized serum TG and glucose, but not cholesterol levels in HFD-fed mice.

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Authors and Affiliations

  1. Skylight Biotech, 100-4 Sunada, Iijima, Akita, 010-0911, Japan

    Jyunichiro Takahashi, Gen Toshima, Yukie Matsumoto & Fumiko Kimura

  2. Harvestech, 3-1-7 Sannou, Akita, 010-0951, Japan

    Takanobu Kiuchi & Kentaro Hamada

  3. Akita Research Institute for Food and Brewing (ARIF), 4-26 Sanuki, Arayamachi, Akita, 010-1623, Japan

    Keishi Hata

Authors
  1. Jyunichiro Takahashi
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  2. Gen Toshima
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  3. Yukie Matsumoto
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  4. Fumiko Kimura
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  5. Takanobu Kiuchi
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  6. Kentaro Hamada
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  7. Keishi Hata
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Corresponding author

Correspondence to Keishi Hata.

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Takahashi, J., Toshima, G., Matsumoto, Y. et al. In vitro screening for antihyperlipidemic activities in foodstuffs by evaluating lipoprotein profiles secreted from human hepatoma cells. J Nat Med 65, 670–674 (2011). https://doi.org/10.1007/s11418-011-0542-x

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  • DOI: https://doi.org/10.1007/s11418-011-0542-x

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