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Journal of Natural Medicines

, Volume 65, Issue 3–4, pp 670–674 | Cite as

In vitro screening for antihyperlipidemic activities in foodstuffs by evaluating lipoprotein profiles secreted from human hepatoma cells

  • Jyunichiro Takahashi
  • Gen Toshima
  • Yukie Matsumoto
  • Fumiko Kimura
  • Takanobu Kiuchi
  • Kentaro Hamada
  • Keishi Hata
Natural Resource Letter

Abstract

We screened the antihyperlipidemic effects of seven edible plants by evaluation of the triglyceride (TG) and cholesterol profiles secreted from HepG2 cells. We found that the water- and ethanol-extracts of Brasenia schreberi at 100 μg/ml exhibited strong inhibitory activities against TG and cholesterol secretions from HepG2 cells stimulated with sodium oleate. Real-time RT-PCR analysis demonstrated that ethanol extract of B. schreberi (BSET) attenuated the expression of the sterol regulatory element binding protein-1c and -2, fatty acid synthase and HMG CoA synthase-1 genes, which are involved in lipid synthesis in hepatocyte/hepatoma cells. Furthermore, we studied the action of BSET on adipose tissue accumulation and serum parameters in mice fed a high-fat diet (HFD). BSET suppressed mesenteric and epididymal adipose tissue accumulation and normalized serum TG and glucose, but not cholesterol levels in HFD-fed mice.

Keywords

HepG2 cell Lipoprotein profile Triglyceride Cholesterol Antihyperlipidemic activity Brasenia schreberi 

Supplementary material

11418_2011_542_MOESM1_ESM.doc (74 kb)
Supplementary table (DOC 74 kb)

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Copyright information

© The Japanese Society of Pharmacognosy and Springer 2011

Authors and Affiliations

  • Jyunichiro Takahashi
    • 1
  • Gen Toshima
    • 1
  • Yukie Matsumoto
    • 1
  • Fumiko Kimura
    • 1
  • Takanobu Kiuchi
    • 2
  • Kentaro Hamada
    • 2
  • Keishi Hata
    • 3
  1. 1.Skylight BiotechAkitaJapan
  2. 2.HarvestechAkitaJapan
  3. 3.Akita Research Institute for Food and Brewing (ARIF)AkitaJapan

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