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Journal of Natural Medicines

, Volume 65, Issue 3–4, pp 417–423 | Cite as

Active components from Siberian ginseng (Eleutherococcus senticosus) for protection of amyloid β(25–35)-induced neuritic atrophy in cultured rat cortical neurons

  • Yanjing Bai
  • Chihiro Tohda
  • Shu Zhu
  • Masao Hattori
  • Katsuko KomatsuEmail author
Original Paper

Abstract

Not only neuronal death but also neuritic atrophy and synaptic loss underlie the pathogenesis of Alzheimer’s disease as direct causes of the memory deficit. Extracts of Siberian ginseng (the rhizome of Eleutherococcus senticosus) were shown to have protective effects on the regeneration of neurites and the reconstruction of synapses in rat cultured cortical neurons damaged by amyloid β (Aβ)(25–35), and eleutheroside B was one of the active constituents. In this study, a comprehensive evaluation of constituents was conducted to explore active components from Siberian ginseng which can protect against neuritic atrophy induced by Aβ(25–35) in cultured rat cortical neurons. The ethyl acetate, n-butanol and water fractions from the methanol extract of Siberian ginseng showed protective effects against Aβ-induced neuritic atrophy. Twelve compounds were isolated from the active fractions and identified. Among them, eleutheroside B, eleutheroside E and isofraxidin showed obvious protective effects against Aβ(25–35)-induced atrophies of axons and dendrites at 1 and 10 μM.

Keywords

Siberian ginseng Eleutherococcus senticosus Amyloid β Neuritic atrophy Eleutheroside B Eleutheroside E 

Notes

Acknowledgments

This work was supported by a Grant-in-Aid for Scientific Research (B), No. 21406004 in 2009–2010, from the Japan Society for the Promotion of Science and by Expansion Program, Regional Innovation Cluster Program, Global Type (II), “Hokuriku Innovation Cluster for Health Science” from the Ministry of Education, Culture, Sports, Science and Technology, Japan.

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Copyright information

© The Japanese Society of Pharmacognosy and Springer 2011

Authors and Affiliations

  • Yanjing Bai
    • 1
  • Chihiro Tohda
    • 2
  • Shu Zhu
    • 1
  • Masao Hattori
    • 1
  • Katsuko Komatsu
    • 1
    Email author
  1. 1.Division of Pharmacognosy, Institute of Natural MedicineUniversity of ToyamaToyamaJapan
  2. 2.Division of Biofunctional Evaluation, Research Center for Ethnomedicine, Institute of Natural MedicineUniversity of ToyamaToyamaJapan

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