Antifibrotic activity of coumarins from Cnidium monnieri fruits in HSC-T6 hepatic stellate cells
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The CHCl3 fraction of Cnidium monnieri fruits significantly inhibited the proliferation of hepatic stellate cells in an in-vitro assay system employing HSC-T6 hepatic stellate cell lines. Activity-guided fractionation of the CHCl3 fraction of C. monnieri led to the isolation of ten coumarins: osthol (1), meranzin (2), auraptenol (3), meranzin hydrate (4), 7-hydroxy-8-methoxy coumarin (5), imperatorin (6), xanthotoxol (7), xanthotoxin (8), bergapten (9) and isopimpinellin (10). Of these, compounds 1 and 6 significantly inhibited proliferation of HSCs in a time- and concentration-dependent manner. In addition, compounds 1 and 6 significantly reduced collagen content in HSC-T6 cells.
KeywordsCnidium monnieri Coumarin HSC-T6 Hepatic stellate cells Osthol Imperatorin
We thank Prof. S.L. Freidman (Columbia University, New York) for the kindly gift of the HSC-T6 cells. This work was supported by the Medical Research Center Program (2010-0029480), the Regional Core Research Program (Chungbuk BIT Research-Oriented University Consortium) and Basic Science Research Program (2010-0025054) through the National Research Foundation of Korea (NRF).
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