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Cytotoxic effect of artocarpin on T47D cells

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Abstract

In our screening projects for anticancer agents from natural resources, artocarpin [6-(3-methyl-1-butenyl)-5,2′,4′-trihydroxy-3-isoprenyl-7-methoxyflavone] isolated from wood of jack fruit (Artocarpus heterophyllus) showed potent cytotoxic activity on human T47D breast cancer cells. The mode of action of artocarpin was evaluated by its effect on cell viability, nuclear morphology, cell cycle progression, expression of protein markers for apoptosis, and mitochondrial membrane potential (Δψm). These results showed that artocarpin caused a reduction of cell viability in a concentration-dependent manner and an alteration of cell and nuclear morphology. Moreover, the percentage of the sub-G1 phase formation was elevated dose-dependently. Artocarpin induced activation of caspase 8 and 10 as indicated by stronger signal intensity of cleaved-caspase 8 and weaker signal intensity of caspase 10 markers detected after artocarpin treatment. In addition, we also noticed the activation of caspase 3 by artocarpin. There were negligible changes in mitochondrial membrane potential (Δψm) due to artocarpin treatment. All together, these data indicated that artocarpin induced apoptosis in T47D cells possibly via an extrinsic pathway.

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Acknowledgments

T47D cells were generously donated by Tjandrawati Mozef, M.Sc., Indonesian Institute of Sciences Research Centre for Chemistry (Natural Products, Food and Pharmaceuticals Division), Bandung, Indonesia.

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Authors and Affiliations

  1. Stem Cell and Cancer Institute, Jalan Ahmad Yani no. 2, Pulomas, Jakarta, 13210, Indonesia

    Enos Tangke Arung, Britanto Dani Wicaksono, Yohana Ayupriyanti Handoko, Dina Yulia & Ferry Sandra

  2. Wood Chemistry Laboratory, Forestry Faculty, Mulawarman University, Jalan KH Dewantara, Kampus Gn. Kelua, Samarinda, East Kalimantan, 75123, Indonesia

    Enos Tangke Arung & Irawan Wijaya Kusuma

  3. Department of Forest and Forest Products Science, Agriculture Faculty, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka, 8128581, Japan

    Kuniyoshi Shimizu

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  1. Enos Tangke Arung
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  2. Britanto Dani Wicaksono
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Correspondence to Ferry Sandra.

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Arung, E.T., Wicaksono, B.D., Handoko, Y.A. et al. Cytotoxic effect of artocarpin on T47D cells. J Nat Med 64, 423–429 (2010). https://doi.org/10.1007/s11418-010-0425-6

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  • DOI: https://doi.org/10.1007/s11418-010-0425-6

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