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Metabolic disease prevention and suppression of fat accumulation by Salacia reticulata

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Abstract

In Ayurvedic medicine, Salacia reticulata is known to be useful against various metabolic diseases, including diabetes and obesity. In this study, we attempted to clarify the antiobesity mechanism and the safety of S. reticulata in vivo and in vitro. We gave ordinary MF feed, alone or mixed with S. reticulata (0.3 or 1.0%), to Tsumura Suzuki obesity diabetes (TSOD) mice (spontaneous obese type II diabetes model mice) and Tsumura Suzuki non-obese (TSNO) mice (the corresponding reference animals), ad libitum for 2 months. As compared with the TSNO control mice, the TSOD control mice became obese due to fat accumulation and developed various signs of metabolic diseases. The TSOD mouse group receiving S. reticulata showed the following effects: suppression of body weight increase and fat accumulation, alleviation of abnormal lipid metabolism and abnormal glucose tolerance, and suppression of intrahepatic fat accumulation. Also, S. reticulata prevented the mesenteric adipocyte hypertrophy recognized in TSOD mice. In the TSNO controls, the feed containing 1.0% S. reticulata exerted a suppressing effect on body weight increase and fat accumulation, but the feed containing 0.3% S. reticulata did not show any effect at all. In an in vitro experiment using mouse-derived adipocyte precursor 3T3-L1 cells, S. reticulata significantly suppressed fat accumulation in the differentiation induction phase and maturation phase. This suggested that the metabolic disease-preventing effects of S. reticulata, including the antiobesity effect, may involve suppression of differentiation and accumulation in the adipocytes.

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References

  1. Matsuzawa Y, Funahashi T, Kihara S, Shimomura I (2004) Adiponectin and metabolic syndrome. Arterioscler Thromb Vasc Biol 24:29–33

    Article  CAS  PubMed  Google Scholar 

  2. Yoshikawa M, Shimoda H, Nishida N, Takada M, Matsuda H (2002) Salacia reticulata and its polyphenolic constituents with lipase inhibitory and lipolytic activities have mild antiobesity effects in rats. J Nutr 132:1819–1824

    CAS  PubMed  Google Scholar 

  3. Yoshikawa M, Ninomiya K, Shimoda H, Nishida N, Matsuda H (2002) Hepatoprotective and antioxidative properties of Salacia reticulata: preventive effects of phenolic constituents on CCl4-induced liver injury in mice. Biol Pharm Bull 25:72–76

    Article  CAS  PubMed  Google Scholar 

  4. Im R, Mano H, Matsuura T, Nakatani S, Shimizu J, Wada M (2009) Mechanisms of blood glucose-lowering effect of aqueous extract from stems of Kothala himbutu (Salacia reticulata) in the mouse. J Ethnopharmacol 121:34–40

    Article  Google Scholar 

  5. Kishino E, Ito T, Fujita K, Kiuchi Y (2009) A mixture of Salacia reticulata (Kotala himbutu) aqueous extract and cyclodextrin reduces body weight gain, visceral fat accumulation, and total cholesterol and insulin increases in male Wistar fatty rats. Nutr Res 29:55–63

    Article  CAS  PubMed  Google Scholar 

  6. Li YH, Peng G, Li Q, Wen SP, Huang THW, Roufogalis BD, Yamahara J (2004) Salacia oblonga improves cardiac fibrosis and inhibits postprandial hyperglycemia in obese zucker rats. Life Sci 75:1735–1746

    Article  CAS  PubMed  Google Scholar 

  7. Huang THW, Peng G, Li GQ, Yamahara J, Roufogalis BD, Li YH (2006) Salacia oblonga root improves postprandial hyperlipidemia and hepatic steatosis in Zucker diabetic fatty rats: activation of PPAR-alpha. Toxicol Appl Pharmacol 210:225–235

    Article  PubMed  Google Scholar 

  8. Ninomiya K, Matsuda H, Shimoda H, Nishida N, Kasajima N, Yoshino T, Morikawa T, Yoshikawa M (2004) Carnosic acid, a new class of lipid absorption inhibitor from sage. Bioorg Med Chem Lett 14:1943–1946

    Article  CAS  PubMed  Google Scholar 

  9. Huang TH, He L, Qin Q, Yang Q, Peng G, Harada M, Qi Y, Yamahara J, Roufogalis BD, Li Y (2008) Salacia oblonga root decreases cardiac hypertrophy in Zucker diabetic fatty rats: inhibition of cardiac expression of angiotensin II type 1 receptor. Diabetes Obes Metab 10:574–585

    Article  CAS  PubMed  Google Scholar 

  10. Matsuda H, Murakami T, Yashiro K, Yamahara J, Yoshikawa M (1999) Antidiabetic principles of natural medicines. IV. Aldose reductase and alpha-glucosidase inhibitors from the roots of Salacia oblonga WALL-(Celastraceae): structure of a new friedelane-type triterpene, kotalagenin 16-acetate. Chem Pharm Bull 47:1725–1729

    CAS  PubMed  Google Scholar 

  11. Im R, Mano H, Nakatani S, Shimizu J, Wada M (2008) Aqueous extract of Kotahla Himbutu (Salacia reticulata) stems promotes oxygen consumption and suppresses body fat accumulation in mice. J Health Sci 54:645–653

    Article  Google Scholar 

  12. Hirayama I, Yi Z, Izumi S, Arai I, Suzuki W, Nagamachi Y, Kuwano H, Takeuchi T, Izumi T (1999) Genetic analysis of obese diabetes in the TSOD mouse. Diabetes 48:1183–1191

    Article  CAS  PubMed  Google Scholar 

  13. Iizuka S, Suzuki W, Tabuchi M, Nagata M, Imamura S, Kobayashi Y, Kanitani M, Yanagisawa T, Kase Y, Takeda S, Aburada M, Takahashi KW (2005) Diabetic complications in a new animal model (TSOD mouse) of spontaneous NIDDM with obesity. Exp Anim 54:71–83

    Article  CAS  PubMed  Google Scholar 

  14. Miura T, Suzuki W, Ishihara E, Arai I, Ishida H, Seino Y, Tanigawa K (2001) Impairment of insulin-stimulated GLUT4 translocation in skeletal muscle and adipose tissue in the Tsumura Suzuki obese diabetic mouse: a new genetic animal model of type 2 diabetes. Eur J Endocrinol 145:785–790

    Article  CAS  PubMed  Google Scholar 

  15. Mizutani S, Gomi H, Hirayama I, Izumi T (2006) Chromosome 2 locus Nidd5 has a potent effect on adiposity in the TSOD mouse. Mamm Genome 17:375–384

    Article  CAS  PubMed  Google Scholar 

  16. Suzuki W, Iizuka S, Tabuchi M, Funo S, Yanagisawa T, Kimura M, Sato T, Endo T, Kawamura H (1999) A new mouse model of spontaneous diabetes derived from ddY strain. Exp Anim 48:181–189

    Article  CAS  PubMed  Google Scholar 

  17. Takahashi A, Tabuchi M, Suzuki W, Iizuka S, Nagata M, Ikeya Y, Takeda S, Shimada T, Aburada M (2006) Insulin resistance and low sympathetic nerve activity in the Tsumura Suzuki obese diabetic mouse: a new model of spontaneous type 2 diabetes mellitus and obesity. Metab Clin Exp 55:1664–1669

    CAS  PubMed  Google Scholar 

  18. Akase T, Shimada T, Harasawa Y, Akase T, Ikeya Y, Nagai E, Iizuka S, Nakagami G (2009) Preventive effects of Salacia reticulata on obesity and metabolic disorders in TSOD mice. Evid Based Complement Alternat Med. doi:10.1093/ecam/nep052

  19. Rong XL, Kim MS, Su N, Wen SP, Matsuo Y, Yamahara J, Murray M, Li YH (2008) An aqueous extract of Salacia oblonga root, a herb-derived peroxisome proliferator-activated receptor-alpha activator, by oral gavage over 28 days induces gender-dependent hepatic hypertrophy in rats. Food Chem Toxicol 46:2165–2172

    Article  CAS  PubMed  Google Scholar 

  20. Ratnasooriya WD, Jayakody J, Premakumara GAS (2003) Adverse pregnancy outcome in rats following exposure to a Salacia reticulata (Celastraceae) root extract. Braz J Med Biol Res 36:931–935

    Article  CAS  PubMed  Google Scholar 

  21. Freedman BD, Lee EJ, Park Y, Jameson JL (2005) A dominant negative peroxisome proliferator-activated receptor-gamma knock-in mouse exhibits features of the metabolic syndrome. J Biol Chem 280:17118–17125

    Article  CAS  PubMed  Google Scholar 

  22. Kishino E, Ito T, Fujita K, Kiuchi Y (2006) A mixture of the Salacia reticulata (Kotala himbutu) aqueous extract and cyclodextrin reduces the accumulation of visceral fat mass in mice and rats with high-fat diet-induced obesity. J Nutr 136:433–439

    CAS  PubMed  Google Scholar 

  23. Shimada T, Kudo T, Akase T, Aburada M (2008) Preventive effects of Bofutsushosan on obesity and various metabolic disorders. Biol Pharm Bull 31:1362–1367

    Article  CAS  PubMed  Google Scholar 

  24. Shimada T, Akase T, Kosugi M, Aburada M (2009) Preventive effect of boiogito on metabolic disorders in the TSOD Mouse, a model of spontaneous obese type II diabetes mellitus. Evid Based Complement Alternat Med. doi:10.1093/ecam/nep012

  25. Furuyashiki T, Nagayasu H, Aoki Y, Bessho H, Hashimoto T, Kanazawa K, Ashida H (2004) Tea catechin suppresses adipocyte differentiation accompanied by down-regulation of PPAR gamma 2 and C/EBP alpha in 3T3–L1 cells. Biosci Biotechnol Biochem 68:2353–2359

    Article  CAS  PubMed  Google Scholar 

  26. Moon HS, Chung CS, Lee HG, Kim TG, Choi YJ, Cho CS (2007) Inhibitory effect of (−)-epigallocatechin-3-gallate on lipid accumulation of 3T3–L1 cells. Obesity 15:2571–2582

    Article  CAS  PubMed  Google Scholar 

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Acknowledgments

This work was partly supported by the “High-Tech Research Center” Project for Private University: matching fund subsidy from MEXT (Ministry of Education Culture, Sports, Science and Technology) 2004–2008 of Japan, and grant-in-aid for Scientific Research (No. 19590703) from MEXT of Japan.

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Authors and Affiliations

  1. Research Institute of Pharmaceutical Sciences, Musashino University, 1-1-20 Shinmachi, Nishitokyo, Tokyo, 202-8585, Japan

    Tsutomu Shimada, Seiichi Iizuka & Masaki Aburada

  2. Department of Clinical Pharmacy, Graduate School of Natural Science and Technology, Kanazawa University, Kakuma-machi, Kanazawa, Ishikawa, 920-1192, Japan

    Eiichi Nagai, Yukiko Harasawa, Takashi Aburada & Ken-ichi Miyamoto

  3. Graduate School of Medicine, Hokkaido University, North-15, West-7, Kita-ku, Hokkaido, 060-8638, Japan

    Eiichi Nagai

  4. Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-0033, Japan

    Tomoko Akase

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  1. Tsutomu Shimada
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  2. Eiichi Nagai
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  3. Yukiko Harasawa
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  4. Tomoko Akase
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  5. Takashi Aburada
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  6. Seiichi Iizuka
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  8. Masaki Aburada
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Corresponding author

Correspondence to Masaki Aburada.

Additional information

T. Shimada and E. Nagai contributed equally to this work.

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Shimada, T., Nagai, E., Harasawa, Y. et al. Metabolic disease prevention and suppression of fat accumulation by Salacia reticulata . J Nat Med 64, 266–274 (2010). https://doi.org/10.1007/s11418-010-0401-1

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  • DOI: https://doi.org/10.1007/s11418-010-0401-1

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