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Antinociceptive effects of the extracts of Xylopia parviflora bark and its alkaloidal components in experimental animals

Journal of Natural Medicines volume 64, pages 9–15 (2010)Cite this article

Abstract

In the present study, we attempted to elucidate the antinociceptive activity of Xylopia parviflora bark using the acetic acid-induced writhing test, hot plate test, and formalin test in mice. The MeOH extract (100 and 200 mg/kg, administered intraperitoneally (i.p.)) had an antinociceptive effect demonstrated by its inhibitory effects on writhing number induced by acetic acid. Three alkaloidal fractions exhibited significant antinociceptive effects in three animal models; the chloroform-soluble fraction, including secondary and tertiary alkaloids, exhibited the strongest effect. This result supported its use in folk medicine as an analgesic agent. We tested the main alkaloids of these fractions for their antinociceptive effects to clarify the active components. (+)-Corytuberine (6.3 and 12.5 mg/kg, i.p.) showed very strong activity, had a significant antinociceptive effect in the acetic acid-induced writhing test (with 49.4 and 98.9% reduction of writhes), in the hot plate test, and in the formalin test (with 55.4 and 90.6% inhibition during the first phase, and 73.9 and 99.9% during the second phase, respectively). (+)-Glaucine (12.5 and 25 mg/kg, i.p.) showed strong activity in three animal models, too. The activity of these compounds was also observed following oral administration in the acetic acid-induced writhing test.

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References

  1. Nishiyama Y, Moriyasu M, Ichimaru M, Iwasa K, Kato A, Mathenge SG, Chalo Mutiso PB, Juma FD (2004) Quaternary isoquinoline alkaloids from Xylopia parviflora. Phytochemistry 65:939–944

    Article  CAS  PubMed  Google Scholar 

  2. Nishiyama Y, Moriyasu M, Ichimaru M, Iwasa K, Kato A, Mathenge SG, Chalo Mutiso PB, Juma FD (2006) Secondary and tertiary isoquinoline alkaloids from Xylopia parviflora. Phytochemistry 67:2671–2675

    Article  CAS  PubMed  Google Scholar 

  3. Kato A, Moriyasu M, Nishiyama Y, Ichimaru M, Juma FD, Ogeto JO (1993) Ion-pair and ion-suppression high performance liquid chromatographic analysis of alkaloial constituents of Artabotrys monteiroae. Phytochem Anal 4:72–75

    Article  CAS  Google Scholar 

  4. Ichimaru M, Moriyasu M, Nishiyama Y, Kato A, Juma FD, Nganga JN, Ogeto JO (1997) Studies on African medicinal plants. Alkaloidal constituents of Uvaria acuminata and U. lucida. Nat Med 51:272–274

    CAS  Google Scholar 

  5. Nishiyama Y, Moriyasu M, Sonoda M, Ichimaru M, Iwasa K, Kato A, Mathenge SG, Juma FD (2000) Isoquinoline alkaloids from Monodora junodii. Nat Med 54:42

    Google Scholar 

  6. Nishiyama Y, Moriyasu M, Ichimaru M, Iwasa K, Kato A, Mathenge SG, Chalo Mutiso PB, Juma FD (2000) Secondary and tertiary isoquinoline alkaloids from Monodora junodii. Nat Med 54:338–341

    CAS  Google Scholar 

  7. Zetler G (1988) Neuroleptic-like, anticonvulsant and antinociceptive effects of aporphine alkaloids: bulbocapnine, corytuberine, boldine and glaucine. Arch Int Pharmacodyn Ther 296:255–281

    CAS  PubMed  Google Scholar 

  8. Moriyasu M, Ichimaru M, Nishiyama Y, Kato A (1993) The acquisition of alkaloids from plant materials by a combination of ion-pair extraction and preparative ion-pair HPLC using sodium perchlorate. Bunseki Kagagu 42:659–665

    CAS  Google Scholar 

  9. Kikuchi T, Kadota S, Yanada K, Watanabe K, Yosizaki M, Kimura M (1981) Studies on the constituents of crude drug “Shin-I” (dried buds of Magnolia salicifolia Maxim). Wakanyaku Shinpojumu [Kiroku] 13:101–104

    Google Scholar 

  10. Kato A, Moriyasu M, Ichimaru M, Nishiyama Y, Juma FD, Nganga JN, Mathenge SG, Ogeto JO (1995) Examination of alkaloidal constituents of Zanthoxylum usambarense by a combination of ion-pair extraction and ion-pair chromatography using sodium perchlorate. Phytochem Anal 6:89–95

    CAS  Article  Google Scholar 

  11. Bispo MD, Mourso RHV, Franzotti EM, Bomfim KBR, de Arrigoni-Blank MF, Moreno MPN, Marchioro M, Antoniolli AR (2001) Antinociceptive and antiedematogenic effects of the aqueous extract of Hyptis pectinata leaves in experimental animals. J Ethnopharmacol 76:81–86

    Article  CAS  PubMed  Google Scholar 

  12. Hanskaar S, Hole K (1987) The formalin test in mice: dissociation between inflammatory and non-inflammatory pain. Pain 30:103–114

    Article  Google Scholar 

  13. Tjolsen A, Berge OG, Hunskaar S, Rosland JH, Hole K (1992) The formalin test: an evaluation of the method. Pain 51:5–17

    Article  CAS  PubMed  Google Scholar 

  14. Shibata M, Ohkubo T, Takahashi H, Inoki R (1989) Modified formalin test—characteristic biphasic pain response. Pain 38:347–352

    Article  CAS  PubMed  Google Scholar 

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Affiliations

  1. Department of Natural Medicinal Chemistry, Kobe Pharmaceutical University, 4-19-1 Motoyamakita-machi, Higashinada-ku, Kobe, 658-8558, Japan

    Yumi Nishiyama, Masataka Moriyasu, Momoyo Ichimaru, Kinuko Iwasa & Atsushi Kato

  2. Department of Botany, University of Nairobi, P.O. Box 30197, Nairobi, Kenya

    Simon G. Mathenge & Patrick B. Chalo Mutiso

  3. Department of Clinical Pharmacology, Kenyatta National Hospital, University of Nairobi, P.O. Box 19676, Nairobi, Kenya

    Francis D. Juma

Authors
  1. Yumi Nishiyama
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  2. Masataka Moriyasu
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  3. Momoyo Ichimaru
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  4. Kinuko Iwasa
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  5. Atsushi Kato
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  6. Simon G. Mathenge
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  7. Patrick B. Chalo Mutiso
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  8. Francis D. Juma
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Correspondence to Yumi Nishiyama.

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Nishiyama, Y., Moriyasu, M., Ichimaru, M. et al. Antinociceptive effects of the extracts of Xylopia parviflora bark and its alkaloidal components in experimental animals. J Nat Med 64, 9–15 (2010). https://doi.org/10.1007/s11418-009-0356-2

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  • DOI: https://doi.org/10.1007/s11418-009-0356-2

Keywords

  • Xylopia parviflora
  • Annonaceae
  • Antinociceptive effect
  • Isoquinoline alkaloids