Abstract
Electron microscopic observation revealed that lupeol induced melanosome maturation in B16 2F2 mouse melanoma cells and we therefore studied the effects of lupeol on the intracellular events responsible for melanosome transport. Incubation with lupeol for 8 h attenuated the actin stress fiber assembly in B16 2F2 mouse melanoma cells, resulting in dendritic formation in the cells. Longer exposure to lupeol (48 h) increased the expression of tyrosinase, MITF (a specific transcription factor for tyrosinase), Rab27a, and myosin-Va, which are required for melanosome transport.
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Abbreviations
- MITF:
-
Microphthalmia-associated transcriptional factor
- TEM:
-
Transmission electron microscopy
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This work was supported in part by a Grant-in-aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology.
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Ogiwara, K., Hata, K. Melanoma cell differentiation induced by lupeol separates into two stages: morphological and functional changes. J Nat Med 63, 323–326 (2009). https://doi.org/10.1007/s11418-009-0319-7
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DOI: https://doi.org/10.1007/s11418-009-0319-7