Journal of Natural Medicines

, Volume 63, Issue 1, pp 9–14 | Cite as

Triterpenes from Cynomorium songaricium—analysis of HCV protease inhibitory activity, quantification, and content change under the influence of heating

  • Chao-Mei Ma
  • Ying Wei
  • Zhi-Gang Wang
  • Masao Hattori
Original Paper


Inhibitory activity of the three major triterpenes from the stems of Cynomorium songaricum—ursolic acid, acetyl ursolic acid, and malonyl ursolic acid hemiester—and their related compounds were tested for their inhibitory activity on HCV protease; malonyl ursolic acid hemiester was the most potent. A HPLC–PAD (photo diode array detector)–MS method was established to quantify the contents of each triterpene in C. songaricum. Using this method, the effect heating had on the contents was also investigated. It was found that among the three triterpenes, the content of malonyl ursolic acid hemiester decreased most quickly during the heating process.


Ursane Ursolic acid Cynomoriaceae Cynomorium songaricum HCV protease 


  1. 1.
    State Administration of Traditional Chinese Medicine of People’s Republic of China (1999) Zhong Hua Ben Cao, vol 5. Shanghai Science and Technology Press, Shanghai, pp 722–724Google Scholar
  2. 2.
    Jiang ZH, Tanaka T, Sakamoto M, Jiang T, Kouno I (2001) Studies on a medicinal parasitic plant: lignans from the stems of Cynomorium songaricum. Chem Pharm Bull 49:1036–1038PubMedCrossRefGoogle Scholar
  3. 3.
    Ma CM, Nakamura N, Miyashiro H, Hattori M, Shimotohno K (1999) Inhibitory effects of constituents from Cynomorium songaricum and related triterpene derivatives on HIV-1 protease. Chem Pharm Bull 47:141–145PubMedGoogle Scholar
  4. 4.
    Chu QC, Tian XH, Lin M, Ye JN (2006) Electromigration profiles of Cynomorium songaricum based on capillary electrophoresis with amperometric detection. J Agric Food Chem 54:7979–7983PubMedCrossRefGoogle Scholar
  5. 5.
    Zhang CZ, Xu XZ, Li C (1996) Fructosides from Cynomorium songaricum. Phytochemistry 41:975–976CrossRefGoogle Scholar
  6. 6.
    Ma CM, Jia SS, Sun T, Zhang YW (1993) Triterpenes and steroidal compounds from Cynomorium songaricum. Yaoxue Xuebao 28:152–155Google Scholar
  7. 7.
    Zhang SZ, Zhang SY (1991) Chemical constituents of Cynomorium songaricum. Zhongguo Yaoxue Zazhi 26:649–651Google Scholar
  8. 8.
    Di DL, Liu YW, Mao XC, Chen JW (2004) Determination of ursolic acid in Cynomorium songaricum by RP-HPLC. Zhongguo Yiyuan Yaoxue Zazhi 24:730–732Google Scholar
  9. 9.
    Ding HW, Wang Y, Wang SY, You WX (1998) Quantitative determination of ursolic acid in Herba Cynomorium songaricum Rupr. by ultraviolet spectrophotometry. Zhongguo Zhongyao Zazhi 23:102–103PubMedGoogle Scholar
  10. 10.
    Hussein G, Nakamura N, Hattori M, Kakiuchi N, Shimotohno K (2000) Inhibitory effects of Sudanese medicinal plant extracts on hepatitis C virus (HCV) protease. Phytother Res 14:510–516PubMedCrossRefGoogle Scholar
  11. 11.

Copyright information

© The Japanese Society of Pharmacognosy and Springer 2008

Authors and Affiliations

  • Chao-Mei Ma
    • 1
  • Ying Wei
    • 1
  • Zhi-Gang Wang
    • 1
  • Masao Hattori
    • 1
  1. 1.Institute of Natural Medicine, University of ToyamaToyamaJapan

Personalised recommendations