Journal of Natural Medicines

, Volume 62, Issue 1, pp 67–70 | Cite as

Isolation and identification of antioxidants from Pedilanthus tithymaloides

  • Pedro M. AbreuEmail author
  • Susan Matthew
  • Tania González
  • Lucie Vanickova
  • David Costa
  • Ana Gomes
  • Marcela A. Segundo
  • Eduarda Fernandes


A bioassay-guided methodology utilizing 2,2-diphenyl-1-picrylhydrazyl (DPPH), the trolox equivalent antioxidant capacity (TEAC), and reducing power assays, as well as an assessment of scavenging properties against O 2 ·− , H2O2, HOCl, ROO·, ·NO, and ONOO were used to find the main antioxidant principles of Pedilanthus tithymaloides (Euphorbiaceae), a shrub used in traditional Cuban medicine. The principles were identified as kaempferol 3-O-β-D-glucopyranoside-6′′-(3-hydroxy-3-methylglutarate), quercitrin, isoquercitrin, and scopoletin. The contents of total phenolics and flavonoids were found to be 76.0 ± 4.8 mg of gallic acid equivalents/g extract and 9.8 ± 0.4 mg of rutin equivalents/g extract, respectively.


Pedilanthus tithymaloides Flavonoid Coumarin Antioxidant activity Anti-inflammatory activity 



REQUIMTE for financial support, Fundação para a Ciência e Tecnologia and Fundo Social Europeu for post-doc (Matthew S) and PhD (Costa D) grants, and Programme Alβan for scholarship E0D033475CU.


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Copyright information

© The Japanese Society of Pharmacognosy and Springer 2007

Authors and Affiliations

  • Pedro M. Abreu
    • 1
    Email author
  • Susan Matthew
    • 1
  • Tania González
    • 3
  • Lucie Vanickova
    • 1
  • David Costa
    • 2
  • Ana Gomes
    • 2
  • Marcela A. Segundo
    • 2
  • Eduarda Fernandes
    • 2
  1. 1.Faculdade de Ciências e Tecnologia da Universidade Nova de LisboaCQFB/REQUIMTECaparicaPortugal
  2. 2.Departamento de Química-Física, Faculdade de Farmácia da Universidade do PortoREQUIMTEPortoPortugal
  3. 3.Departamento de Farmacia, Facultad de Ciencias NaturalesUniversidad de OrienteSantiago de CubaCuba

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