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Inhibitory effect of oral intake of natural Phellinus linteus fruit body on growth and pulmonary metastasis of B16/BL6 melanoma

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Abstract

In the present study, the immunotherapeutic activity of oral intake of natural Phellinus linteus fruit body (nPF) against growth and pulmonary metastasis of injected B16/BL6 melanoma cells has been examined. The nPF powder was orally administered to B16/BL6-bearing mice, a model for the pulmonary metastasis of melanoma, at doses of 83, 415 and 2,075 mg/kg day in their diet. The dietary intake of nPF powder significantly prolonged the survival time of the B16/BL6-bearing mice. Furthermore, ingestion of nPF dose-dependently enhanced NO production by macrophages prepared from the tumor-bearing, nPF-treated animals. Simultaneously, nPF did not induce direct cytotoxicity against B16/BL6. These results suggest that the oral intake of nPF powder may be useful in immunochemotherapy for cancer because of its life prolongation through the inhibitory effect on growth and metastasis of cancer.

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Authors and Affiliations

  1. Laboratory of Pharmacognosy, Graduate School of Pharmaceutical Sciences, Nagoya City University, 3-1 Tanabe-dori, Mizuhoku, Nagoya, 467-8603, Japan

    Takamasa Ohno, Yurika Takahashi, Hiroki Tanabe, Hajime Mizukami & Makoto Inoue

  2. Laboratory of Medicinal Resources, School of Pharmacy, Aichi Gakuin University, 1-100 Kusumoto-cho, Chikusa-ku, Nagoya, 464-8650, Japan

    Takamasa Ohno, Hiroki Tanabe & Makoto Inoue

  3. Nihon Shoyaku Co. Ltd., Rokuban-cho 2, Chiyoda-ku, Tokyo, 158-8501, Japan

    Hideki Hirayama

  4. Nihon Pharmaceutical University, 10281 Komuro, Ina-cho, Kita-adachi-gun, Saitama, 362-0806, Japan

    Yukio Ogihara

Authors
  1. Takamasa Ohno
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  2. Yurika Takahashi
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  3. Hiroki Tanabe
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  4. Hideki Hirayama
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  5. Hajime Mizukami
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  6. Yukio Ogihara
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  7. Makoto Inoue
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Correspondence to Makoto Inoue.

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Ohno, T., Takahashi, Y., Tanabe, H. et al. Inhibitory effect of oral intake of natural Phellinus linteus fruit body on growth and pulmonary metastasis of B16/BL6 melanoma. J Nat Med 61, 438–442 (2007). https://doi.org/10.1007/s11418-007-0163-6

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  • DOI: https://doi.org/10.1007/s11418-007-0163-6

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