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Flavonoid metabolites in urine after oral administration of aqueous extract of Persea americana to rats

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Abstract

In the present study, urinary excretion of the flavonoid metabolites of Persea americana was investigated. Aqueous extract of the leaves of P. americana was administered by gastric probe to Sprague–Dawley rats and urine samples were collected daily for five days. The urine samples were analyzed by reversed-phase HPLC–UV detection. The result obtained showed extensive degradation of the flavonoid of P. americana within the first day. The phenylalkyl acids detected, 3,4-dihydroxyphenylacetic acid (I), hippuric acid (II), 3-hydroxyphenylacetic acid (III), 3-methoxy-4-hydroxyphenylacetic acid (IV), 3-(4-hydroxyphenyl)propionic acid (V) and benzoic acid (VI) were confirmed by their HPLC retention times or by cochromatography with reference standards. No flavonoid glycosides or aglycones were detected in the urine samples.

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Acknowledgments

The authors are thankful to Messers Ojo and Owolabi for the animal care, and the College of Medicine, University of Lagos, Lagos for allowing the use of the Central Research Laboratory.

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Authors and Affiliations

  1. Department of Pharmaceutical Chemistry, Faculty of Pharmacy, College of Medicine Campus, University of Lagos, Lagos, Nigeria

    Mbang A. Owolabi & Herbert A. B. Coker

  2. Department of Physiology, College of Medicine, Idi-Araba, University of Lagos, P.M.B. 12003, Lagos, Nigeria

    Smith I. Jaja

Authors
  1. Mbang A. Owolabi
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  2. Herbert A. B. Coker
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  3. Smith I. Jaja
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Correspondence to Mbang A. Owolabi.

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Owolabi, M.A., Coker, H.A.B. & Jaja, S.I. Flavonoid metabolites in urine after oral administration of aqueous extract of Persea americana to rats. J Nat Med 61, 200–204 (2007). https://doi.org/10.1007/s11418-006-0122-7

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  • DOI: https://doi.org/10.1007/s11418-006-0122-7

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