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Anticancer properties of panduratin A isolated from Boesenbergia pandurata (Zingiberaceae)

Journal of Natural Medicines volume 61, pages 131–137 (2007)Cite this article

Abstract

Extract of Boesenbergia pandurata (Kaempferia pandurata) (Zingiberaceae) has been used as a replacement for K. rotunda, the main ingredient of a popular traditional tonic called “jamu” especially for women in Indonesia. From our previous study, ethanolic extract of B. pandurata showed strong inhibitory effects on the growth of cancer cells, similar to ethanolic extract of Curcuma longa. C. longa and its bioactive compound, curcumin, have shown potential anticancer activity in in vitro and in vivo studies and have undergone clinical trials. Panduratin A, a chalcone derivative isolated from B. pandurata, was found to inhibit the growth of MCF-7 human breast cancer and HT-29 human colon adenocarcinoma cells with an IC50 of 3.75 and 6.56 µg/ml, respectively. Panduratin A arrested cancer cells labelled with Annexin-V and propidium iodide in the G0/G1 phase and induced apoptosis in a dose-dependent manner. In an animal model study, male Sprague–Dawley rats were fed with AIN diet containing ethanolic extracts prepared from the equivalent of 4% by weight of dried rhizomes of B. pandurata and C. longa. Aberrant crypt foci (ACFs) were induced by two subcutaneous doses (15 mg/kg body weight) of azoxymethane (AOM) 1 week apart. The rats were killed 10 weeks later, and the ACFs were assessed in the colon. At the dose given to rats, it appeared that the extracts were not toxic. Total ACFs were slightly reduced by B. pandurata extract compared to control group but not significantly different. Extract of B. pandurata may have a protective effect against colon cancer but additional studies using different models, such as a breast cancer model, need to be carried out.

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Acknowledgements

The authors thank Dr Yoji Hayasaka of the Australian Wine Research Institute for LC-MS data, Phil Clements of the Department of Chemistry at the University of Adelaide for NMR data and Dr Lindsay Dent of the Department of Molecular Biosciences of the University of Adelaide for permission to use the FACScan equipment. This project was funded by AusAID and The Adelaide University and CSIRO Collaborative Grant Program 2000.

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Authors and Affiliations

  1. CSIRO Human Nutrition, P.O. Box 10041, Adelaide BC, SA, Australia, 5000

    Chandra Kirana, Ian Roland Record & Graeme Howie McIntosh

  2. Department of Biology, Faculty of Mathematics and Natural Sciences, Brawijaya University, Malang, Indonesia

    Chandra Kirana

  3. Faculty of Science, School of Agriculture, Food and Wine, Adelaide University, Adelaide, SA, Australia

    Graham Peter Jones

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  1. Chandra Kirana
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  2. Graham Peter Jones
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  3. Ian Roland Record
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  4. Graeme Howie McIntosh
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Correspondence to Chandra Kirana.

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Kirana, C., Jones, G.P., Record, I.R. et al. Anticancer properties of panduratin A isolated from Boesenbergia pandurata (Zingiberaceae). J Nat Med 61, 131–137 (2007). https://doi.org/10.1007/s11418-006-0100-0

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  • DOI: https://doi.org/10.1007/s11418-006-0100-0

Keywords

  • Panduratin A
  • Boesenbergia pandurata
  • Zingiberaceae
  • Apoptosis
  • Cell cycle arrest
  • Azoxymethane
  • Aberrant crypt foci