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Journal of Natural Medicines

, Volume 60, Issue 3, pp 225–230 | Cite as

Ephedrae herba (Mao) decreased histamine content in RBL-2H3 cells

Original Paper
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Abstract

The effect of Ephedrae herba (Mao) on histamine content was investigated. When rat basophilic leukemia (RBL-2H3) cells were incubated for 48 h with Mao, Mao decreased histamine content in a concentration-dependent manner. However, the ratio of released histamine to total histamine was scarcely affected by Mao treatment when RBL-2H3 cells were stimulated by ionomycin. On the other hand, the content of β-hexosaminidase, another marker of degranulation, was only slightly decreased by Mao. The expression level of the active form (53 kDa) of l-histidine decarboxylase, the enzyme which synthesizes histamine, was partially suppressed by Mao. Furthermore, Mao significantly suppressed proliferation of RBL-2H3 cells in a concentration-dependent manner. The phosphodiesterase inhibitor 3-isobutyl-1-methylxanthine revealed an increasing effect of Mao on cAMP level in RBL-2H3 cells. Since this result suggested that Mao may stimulate adenylate cyclase, we evaluated the effect of adenylate cyclase inhibitor SQ 22536 on Mao-induced decrease in histamine content. As a result, we showed that SQ 22536 did not have a reducing effect of Mao. From these results it is understood that Mao decreased histamine content by inhibiting cell proliferation and expression of histidine decarboxylase. However, these effects are not related to the increase in cAMP.

Keywords

Ephedrae herba l-Histidine decarboxylase RBL-2H3 cell Histamine content cAMP 

References

  1. 1.
    Metcalfe DD, Baram D, Mekori YA (1997) Mast cells. Physiol Rev 77:1033–1079CrossRefPubMedGoogle Scholar
  2. 2.
    Kinet JP (1999) The high-affinity IgE receptor (FcεRI): from physiology to pathology. Annu Rev Immunol 17:931–972CrossRefPubMedGoogle Scholar
  3. 3.
    Tkaczyk C, Gilfillan AM (2001) FcεRI-dependent signaling pathways in human mast cells. Clin Immunol 99:198–210CrossRefPubMedGoogle Scholar
  4. 4.
    Hart PH (2001) Regulation of the inflammatory response in asthma by mast cell products. Immunol Cell Biol 79:149–153CrossRefPubMedGoogle Scholar
  5. 5.
    Seldin DC, Adelman S, Austen KF, Stevens RL, Hein A, Caulfield JP, Woodbury RG (1985) Homology of the rat basophilic leukemia cell and the rat mucosal mast cell. Proc Natl Acad Sci USA 82:3871–3875CrossRefPubMedPubMedCentralGoogle Scholar
  6. 6.
    Ukai K, Taya M, Sakakura Y, Sakakura K, Hamaguchi H, Ito Y, Kubo M, Murai S (1990) Clinical evaluation of Mao-bushi-saishin-to extract for perennial nasal allergy (abstract in English). ORL Clinic 83:155–165Google Scholar
  7. 7.
    Ito H, Baba S, Takagi I, Nishimura J, Iwata S, Seno T, Ishihara M, Nishimura T, Yagisawa M, Matsunaga J, Koide A, Tsukiyama M, Sugiyama K, Yokota A, Ito H, Oya Y, Kawai T, Kunii H, Ito Y, Itaya T, Kato S, Hojo S, Isaji H, Ito H, Itaya S, Naito T, Suzuki T (1991) Clinical studies on the prophylactic effect of Chinese herbal medicine (Mao-Bushi-Saishin-To) on Japanese cedar pollinosis (abstract in English). Pract Otol (Kyoto) 52(Suppl):89–106CrossRefGoogle Scholar
  8. 8.
    Ohashi Y, Nakai Y, Furuya H, Esaki Y, Hachikawa K, Tamura T (1992) Clinical effect of Mao-Bushi-Saishin-to in patients with obstinate nasal blockage due to perennial rhinitis (abstract in English). Pract Otol (Kyoto) 85:1845–1853CrossRefGoogle Scholar
  9. 9.
    Saito S, Maruyama Y, Kamiyama S, Nakahata N, Ohizumi Y (2004) Ephedrae herba in Mao-Bushi-Saishin-to inhibits IgE-mediated histamine release and increases cAMP content in RBL-2H3 cells. J Pharmacol Sci 95:41–46CrossRefPubMedGoogle Scholar
  10. 10.
    Saito S, Kamiyama S, Oda M, Nakahata N, Ohizumi Y (2004) Inhibitory effect of Mao-Bushi-Saishin-to on prostaglandin E2 synthesis in C6 rat glioma cells. Biol Pharm Bull 27:1133–1135CrossRefPubMedGoogle Scholar
  11. 11.
    Takagi Y, Higashi N, Kawai S, Maeda A, Ueba N (2004) Effects of traditional oriental medicine on influenza virus infection: activating effect of Mao-Bushi-Saishin-to (MBST, Ma-Huang-Fu-Zi-Xin-Tang) on the function of alveolar macrophages in aged mice. Int Congr Ser 1263:503–506CrossRefGoogle Scholar
  12. 12.
    Tanaka S, Nemoto K, Yamamura E, Ichikawa A (1998) Intracellular localization of the 74- and 53-kDa forms of l-histidine decarboxylase in a rat basophilic/mast cell line, RBL-2H3. J Biol Chem 273:8177–8182CrossRefPubMedGoogle Scholar

Copyright information

© The Japanese Society of Pharmacognosy and Springer 2006

Authors and Affiliations

  • Shin-ya Saito
    • 1
  • Sachie Kamiyama
    • 1
  • Yasushi Ohizumi
    • 1
  1. 1.Department of Pharmaceutical Molecular Biology, Graduate School of Pharmaceutical SciencesTohoku UniversitySendai 980-8578Japan

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