Immunomodulatory effect and quantitation of a cytotoxic glycosphingolipid from Murdannia loriformis
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NMR signal reassignments for a cytotoxic glycosphingolipid compound, 2, β-O-D-glucopyranosyl-2-(2′-hydroxy-Z-6′-enecosamide)sphingosine, isolated from an ethanolic extract of the herb Murdannia loriformis, have been achieved by use of FAB-MS, and 1D and 2D 1H and 13C NMR. The amount of 2 in the herb juice was quantitatively determined by use of a validated HPLC method (RP-18, MeOH–H2O, UV detection at 210 nm). The immunomodulatory effect of the herb juice and of 2 was proved by means of in vitro cellular immunological assays. Compound 2 at a concentration of 13 nmol L−1 stimulated PBMC proliferation and increased the CD 3,4:CD 3,8 ratio in T lymphocytes.
KeywordsMurdannia loriformis Commelinaceae Cytotoxic glycosphingolipid β-O-D-glucopyranosyl-2-(2′-hydroxy-Z-6′-enecosamide)sphingosine Quantitative HPLC analysis Immunomodulator
The authors thank the NRCT-JSPS, the Government Pharmaceutical Organization of Thailand and Mahidol University, Bangkok, Thailand, for financial support. Jiratchariyakul W is very grateful for the EU postdoctoral fellowship at the Department of Pharmaceutical Chemistry, Institute of Pharmacy, Freiburg University, Germany.
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