Distinction of water-soluble constituents between some Paecilomyces (= Isaria) and Cordyceps fungi by capillary electrophoresis
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In order to reveal the differences between Cordyceps, Paecilomyces (= Isaria) and Nomuraea, we collected seven entomogenous fungi grown in natural field and analyzed the profiles of water-soluble constituents derived from some different sources of Cordyceps, Paecilomyces and Nomuraea by determination using capillary electrophoresis. C. sinensis and C. kyushuensis showed similar peak clusters of protein migrated at 5–7, 8–9, and 12–20 min. The peak clusters obtained from N. atypicola was similar to those of C. sinensis and C. kyushuensis. The water-soluble constituent clusters of C. militaris migrated at 5–9 and 10–15 min were partly different from those of other Cordyceps. It was also revealed that the P. tenuipes and P. cicadae showed lesser peak clusters rather than Cordyceps. These results indicated that the profiles of protein of these entomogenous fungi by capillary electrophoretic analysis could serve as fingerprints for classification and medicinal quality control of Cordyceps.
KeywordsCordyceps Paecilomyces Nomuraea Entomogenous fungus Capillary electrophoresis Fingerprints
- 5.Takano F, Yahagi N, Yahagi R, Takada S, Yamaguchi M, Shoda S, Murase T, Fushiya S, Ohta T (2005) The liquid culture filtrates of Paecilomyces tenuipes (Peck) Samson (= Isaria japonica Yasuda) and Paecilomyces cicadae (Miquel) Samson (= Isaria sinclairii (Berk.) Llond) regulate Th1 and Th2 cytokine response in murine Peyer’s patch cells in vitro and ex vivo. Int Immunopharmacol 5:903–916CrossRefPubMedGoogle Scholar
- 6.Shiao MS, Wang ZN, Lin LJ, Lien JY, Wang JJ (1994) Profiles of nucleosides and nitrogen bases in Chinese medicinal fungus Cordyceps sinensis and related species. Bot Bull Acad Sinica 35:261–267Google Scholar
- 9.Samson RA (1974) Paecilomyces and some allied hyphomycetes. Stud Mycol 6:1–119Google Scholar
- 10.Hatamori Y, Kaneko S (1998) Notes on teleomorph of Nomuraea atypicola (in Japanese). J Jpn Assoc Cordyceps (Tochukaso) 18:25–32Google Scholar