Journal of Natural Medicines

, Volume 60, Issue 3, pp 240–242 | Cite as

Isolation of cytochrome P450 3A (CYP3A) inhibitors from Hyuganatsu, Citrus tamurana Hort.

  • Shinzo Hosoi
  • Eri Shimizu
  • Noriyuki Usami
  • Ikuo Yamamoto
  • Kazuhiko Arimori
  • Manabu Okumura
  • Muneaki Hidaka
  • Mitsuko Yamada
  • Akiyo Sakushima
Note

Abstract

The present study was conducted to identify cytochrome P450 3A (CYP3A) inhibitory components of Hyuganatsu, Citrus tamurana Hort., by investigating the effects on midazolam 1′-hydroxylase activity of human liver microsomes. As a consequence, limonin and nomilin were identified as CYP3A inhibitors from the endocarp of Hyuganatsu.

Keywords

CYP 3A Hyuganatsu Limonoid Citrus tamurana Enzyme inhibitor 

Notes

Acknowledgements

This work was financially supported by the Promotion of Evolutional Science and Technology in Miyazaki Prefecture from the Miyazaki 21st Century Strategy Promotion Foundation. The authors thank Prof. Keiji Wada, Health Sciences University of Hokkaido, and Associate Prof. Yasuhiro Tezuka, Toyama Medical and Pharmaceutical University for the generous gift of nomilin and its NMR spectra, respectively.

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Copyright information

© The Japanese Society of Pharmacognosy and Springer 2006

Authors and Affiliations

  • Shinzo Hosoi
    • 1
  • Eri Shimizu
    • 1
  • Noriyuki Usami
    • 1
  • Ikuo Yamamoto
    • 1
  • Kazuhiko Arimori
    • 2
  • Manabu Okumura
    • 2
  • Muneaki Hidaka
    • 2
  • Mitsuko Yamada
    • 3
  • Akiyo Sakushima
    • 1
  1. 1.School of Pharmaceutical SciencesKyushu University of Health and WelfareNobeoka 882-8508Japan
  2. 2.Department of PharmacyMiyazaki Medical College Hospital Miyazaki 889-1692Japan
  3. 3.Department of Nutrition Science, Faculty of Health and Nutrition SciencesMinami Kyushu UniversityMiyazaki 880-0032Japan

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