Isolation of cytochrome P450 3A (CYP3A) inhibitors from Hyuganatsu, Citrus tamurana Hort.
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The present study was conducted to identify cytochrome P450 3A (CYP3A) inhibitory components of Hyuganatsu, Citrus tamurana Hort., by investigating the effects on midazolam 1′-hydroxylase activity of human liver microsomes. As a consequence, limonin and nomilin were identified as CYP3A inhibitors from the endocarp of Hyuganatsu.
KeywordsCYP 3A Hyuganatsu Limonoid Citrus tamurana Enzyme inhibitor
This work was financially supported by the Promotion of Evolutional Science and Technology in Miyazaki Prefecture from the Miyazaki 21st Century Strategy Promotion Foundation. The authors thank Prof. Keiji Wada, Health Sciences University of Hokkaido, and Associate Prof. Yasuhiro Tezuka, Toyama Medical and Pharmaceutical University for the generous gift of nomilin and its NMR spectra, respectively.