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Identification of the main volatile compounds in the leaf and flower of Tithonia diversifolia (Hemsl) Gray


The composition of the essential oils of the leaves and flowers of Tithonia diversifolia (Hemsl) A. Gray, Mexican sunflower, are reported. The oils were obtained by hydrodistillation in an all-glass Clevenger-type apparatus and analyzed by GC–MS. The leaf oil was comprised of an abundance of α-pinene (32.9%), β-caryophyllene (20.8%), germacrene D (12.6%), β-pinene (10.9%) and 1, 8-cineole (9.1%). Germacrene D (20.3%), β-caryophyllene (20.1%) and bicyclogermacrene (8.0%) characterized the oil of the flower. A number of aliphatic fatty acids and a diterpenoid compound, sandaracopimaradiene, that were present in the flower, could not be detected in the leaf oil.

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  1. 1.

    Elufioye TO, Agbedahunsi JM (2004) Antimalarial activities of Tithonia diversifolia (Asteraceae) and Crossopteryx febrifga (Rubiaceae) on mice in vivo. J Ethnopharmacol 93:161–171

    Article  Google Scholar 

  2. 2.

    Owoyele VB, Wuraola CO, Soladoye AO, Olaleye SB (2004) Studies on the anti-inflammatory and analgesic properties of Tithonia diversifolia leaf extract. J Ethnopharmacol 90:317–321

    PubMed  Article  Google Scholar 

  3. 3.

    Cos P, Hermans N, De Bruyne T, Apers S, Sindambiwe JB, Witvrouw M, De Clercq E, Vanden Berghe D, Pieters L, Vlietinck AJ (2002) Antiviral activity of Rwandan medicinal plants against human immunodeficiency virus type-1. Phytomedicine 9:62–68

    PubMed  CAS  Article  Google Scholar 

  4. 4.

    Tona L, Kambu K, Ngimbi N, Cimanga K, Vlietnick AJ (1998) Antiamoebic and phytochemical screening of some Congolese medicinal plants. J Ethnopharmacol 61:57–65

    PubMed  CAS  Article  Google Scholar 

  5. 5.

    Elufioye TO, Agbedahunsi JM (2004) 8-(2-methylbutanoyl)-3, 10- epoxy-3, 8-dihydroxyl-4, 11 (13)-germacradien-12, 6-olide, a new sesquiterpene lactone from Tithonia diversifolia. Nig J Nat Prod Med 8:74–76

    CAS  Google Scholar 

  6. 6.

    Gu JQ, Park EJ, Totura S, Riswan S, Fong HHS, Pezzuto JM, Kinghorn AD (2002) Sesquiterpenoids from Tithonia diversifolia with potential cancer chemopreventive activity. J Nat Prod 65:532–536

    PubMed  CAS  Article  Google Scholar 

  7. 7.

    Kuo YH, Chen C H (1997) Diversifolol, a novel rearranged eudesmane sesquiterpene from the leaves of Tithonia diversifolia. Chem Pharm Bull 45:1223–1224

    CAS  Google Scholar 

  8. 8.

    Rüngeler P, Lyb G, Castro V, Mora G, Pahl HL, Merfort L (1998) Study of three sesquiterpene lactones from Tithonia diversifolia on their anti-inflammatory activity using the transcription factor NF–kB and enzymes of the arachidonic pathways as targets. Planta Med 64:588–593

    PubMed  Google Scholar 

  9. 9.

    Wu T-S, Shi LS, Kuo PC, Leu YL, Liou MJ, Wu PL, Wu YL, Iou SC, Chen YP, Chang HC (2001) Cytotoxic principles from the leaves of Tithonia diversifolia. Chin Pharm J 53:217–223

    CAS  Google Scholar 

  10. 10.

    Lamaty G, Menut C, Zollo PHA, Kuiate JR, Bessiere J-M, Kodou J (1991) Aromatic plants of tropical central Africa. III. Constituents of the essential oil of the leaves of Tithonia diversifolia (Hemsl) Gray from Cameroon. J Essent Oil Res 3:399–402

    CAS  Google Scholar 

  11. 11.

    Menut C, Lamaty G, Zollo PHA, Kuiate JR, Bessiere J-M, Amvam-Zollo PH (1992) Aromatic plants of tropical central Africa. IX. Chemical composition of flower essential oils of Tithonia diversifolia (Hemsl) A. Gray from Cameroon. J Essent Oil Res 4:651–653

    CAS  Google Scholar 

  12. 12.

    Mozuraitis R, Stranden M, Ramirez MI, Borg-Karlson A.-K, Mustaparta H. (2002) (−)-Germacrene D increases attraction and oviposition by the tobacco budworm moth Heliothis virescens. Chem Senses 27:505–509

    PubMed  CAS  Article  Google Scholar 

  13. 13.

    Hmamouchi M, Hmamouchi J, Zouhdi M (2005) Chemical and antimicrobial properties of essential oils of five Moroccan Pinaceae. In: Lawrence BM (ed) The antimicrobial/biological activity of essential oils. Allured, Carol Stream, IL, pp 368–372

  14. 14.

    Martin S, Padilla E, Ocete MA, Galvez J. Jimenez J, Zarzuelo A (1993) Anti-inflammatory activity of the essential oil of Bulpleurum frutescescens. Planta Med 59:533–536

    PubMed  CAS  Google Scholar 

  15. 15.

    Grujic-Jovanovic D, Skaltsa HD, Marin P, Sokovic M (2004) Composition and antibacterial activity of the essential oil of six Stachys species from Serbia. Flav Fragr J 19:139–144

    CAS  Article  Google Scholar 

  16. 16.

    Lago JHG, de Ávila Jr P, de Aquino EM, Moreno PRH, Ohara MT, Limberger RP, Apel MA, Henriques AT (2004) Volatile oils from leaves and stem barks of Cedrela fissilis (Meliaceae): chemical composition and antibacterial activities. Flav Fragr J 19:448–451

    CAS  Article  Google Scholar 

  17. 17.

    Davis PH, Tabanca N, Demirci F, Ozek T, Tumen G, Baser KHC (2001) Composition and antimicrobial activity of the essential oil of Origanum × dolichosiphon. Chem Nat Comp 37:238–241

    Article  Google Scholar 

  18. 18.

    Esteves I, Souza IR, Rodrigues M, Cardoso LGV, Silva Santos LS, Sertie JAA, Perazzo FF, Lima LM, Schneedorf JM, Bastos JK, Carvalho JCT (2005) Gastric antiulcer and anti-inflammatory activities of the essential oil from Casearia sylvestris Sw. J Ethnopharmacol 101:191–196

    PubMed  CAS  Article  Google Scholar 

  19. 19.

    Adams RP (1995) Identification of essential oil components by gas chromatography/mass spectrometry. Allured, Carol Stream, IL

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Correspondence to Dorcas O. Moronkola.

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Moronkola, D.O., Ogunwande, I.A., Walker, T.M. et al. Identification of the main volatile compounds in the leaf and flower of Tithonia diversifolia (Hemsl) Gray. J Nat Med 61, 63–66 (2007).

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  • Tithonia diversifolia (Hemsl) A. Gray
  • Volatile oil
  • GC–MS
  • α-Pinene
  • β-Caryophyllene
  • Germacrene D