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Effects of Eucommia ulmoides Oliver leaf extract on 3T3-L1 differentiation into adipocytes

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Abstract

The extract prepared from roasted Eucommia ulmoides Oliver leaves, Du-Zhong tea, was examined to explore its effect on differentiation of mouse 3T3-L1 preadipocyte cells into adipocytes. The boiling water extract of Du-Zhong tea inhibited lipid accumulation in 3T3-L1. The HPLC analysis of the extract identified catechin, protocatechuic acid, pyrogallol, and chlorogenic acid. Catechin weakly inhibited lipid accumulation after 3T3-L1 differentiation, while protocatechuic acid and chlorogenic acid showed almost no effect. The activity guided separation of Du-Zhong tea lead the isolation of 5-hydroxymethyl-2-furaldehyde (HMF). HMF inhibited lipid accumulation at a concentration of 100 μM, and the amount of lipid in the cells was reduced to the similar level of negative control. This is the first isolation of HMF from Du-Zhong tea and the first observation of its effect on 3T3-L1 differentiation.

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Acknowledgements

We thank Chisato Ono for the excellent technical assistance. This work was supported in part by a grant-in-aid for scientific research (16580089) to N.M. from the Japanese Society for the Promotion of Science.

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Authors and Affiliations

  1. Laboratory of Bio-organic Chemistry, Akita Prefectural University, 010-0195, Akita, Japan

    Eriko Matsuda, Yuko Yoshizawa, Yuki Yokosawa, Naomi Watanabe & Noboru Murofushi

  2. Akita Prefectural Institute of Public Health, Akita, Japan

    Eriko Matsuda

  3. Laboratory of Bio-organic Chemistry, Tokyo Denki University, Saitama, Japan

    Satoru Kawaii

Authors
  1. Eriko Matsuda
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  2. Yuko Yoshizawa
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  3. Yuki Yokosawa
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  4. Naomi Watanabe
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  5. Satoru Kawaii
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  6. Noboru Murofushi
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Correspondence to Yuko Yoshizawa.

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Matsuda, E., Yoshizawa, Y., Yokosawa, Y. et al. Effects of Eucommia ulmoides Oliver leaf extract on 3T3-L1 differentiation into adipocytes. J Nat Med 60, 126–129 (2006). https://doi.org/10.1007/s11418-005-0026-y

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  • DOI: https://doi.org/10.1007/s11418-005-0026-y

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