Abstract
The effect of Daikenchuto (DKT), a traditional Japanese herbal medicine (Kampo medicine), and its constituents (ginger rhizome, ginseng root, rice gluten and Zanthoxylum fruit) on the memory formation process was examined in mice by means of a Morris water maze test. The administration of DKT [300–4000 mg/kg, administered orally (p.o.)] for 3 consecutive days dose-dependently shortened the time required by the mice to find the platform in the water maze test relative to the control. Among the four constituents of DKT, the extract of Zanthoxylum fruit (70 mg/kg, the dose equivalent to 4000 mg/kg DKT) administered p.o. for 3 consecutive days significantly promoted the memory and learning rate. The memory- and learning-enhancing effect was potently elicited by 5 mg/kg (p.o., 2 days) hydroxy-sanshool, the active component of the ethyl acetate fraction of Zanthoxylum fruit. In another series of experiments with the water maze test, the administration of scopolamine [1 mg/kg, intraperitoneally (i.p.)] for 3 consecutive days significantly prolonged the time needed by the mice to find the platform. The subsequent administration of DKT (4000 mg/kg, p.o.) for 3 consecutive days possessed an abatement effect on the scopolamine-induced dementia. The present results indicate that DKT and, more specifically, its constituent Zanthoxylum fruit and the active component of Zanthoxylum fruit, hydroxy-sanshool, all have a memory- and learning-enhancing effect and are probably associated with the release of acetylcholine from neuronal terminals in the brain.
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References
Horie Y, Kato K, Hamano K (1995) An overview of our surgery-oriented kampo. J Tokyo Women’s Med Coll 65:43–51
Furukawa Y, Shiga N, Hanyu N, Hashimoto Y, Mukai H, Nishimura K, Aoki T (1995) Effect of Chinese herbal medicine on gastrointestinal motility and bowel obstruction (in Japanese with English abstract). Jpn J Gastroenterol Surg 28:956–960
Hayakawa T, Kase Y, Saito K, Hashimoto K, Ishige A, Komatsu Y, Sasaki H (1999) Pharmacological studies of the effect of Daikenchuto on spontaneous contraction of isolated rabbit jejunum. J Smooth Muscle Res 35:47–54
Satoh K, Hashimoto K, Hayakawa T, Ishige A, Kaneko M, Ogihara S, Kurosawa S, Yakabi K, Nakamura T (2001) Mechanism of atropine-resistant contraction induced by Daikenchuto in guinea pig ileum. Jpn J Pharmacol 86:32–37
Nakamura T, Sakai A, Isogami I, Noda K, Ueno K, Yano S (2002) Abatement of morphine-induced slowing in gastrointestinal transit by Daikenchuto, a traditional Japanese herbal medicine. Jpn J Pharmacol 88:217–221
Jerusalinsky D, Kornisiuk E, Izquierdo I (1997) Cholinergic neurotransmission and synaptic plasticity concerning memory processing. Neurochem Res 22:507–515
Morris RGM (1984) Developments of a water-maze procedure for studying spatial learning in the rat. J Neurosci Methods 11:47–60
8 Deutsch JA (1971) The cholinergic synapse and the site of memory. Science 174:788–794
Blokland A (1995) Acetylcholine: a neurotransmitter for learning and memory. Brain Res Rev 21:285–300
Deutsch JA, Rocklin KW (1967) Amnesia induced by scopolamine and its temporal variations. Nature 216:89–90
Hashimoto K, Satoh K, Kase Y, Ishige A, Kubo M, Sasaki H, Nishikawa S, Kurosawa S, Yakabi K, Nakamura T (2001) Modulatory effect of aliphatic acid amides from Zanthoxylum piperitum on isolated gastrointestinal tract. Planta Med 67:179–181
Chopin P, Colpaert FC, Marien M (2002) Effects of acute and subchronic administration of dexefaroxan, an alpha(2)-adrenoceptor antagonist, on memory performance in young adult and aged rodents. J Pharmacol Exp Ther 301:187–196
Tottori K, Nakai M, Uwamoto Y, Miwa T, Yamada S, Oshino Y, Kikuchi T, Alter CA (2002) Attenuation of scopolamine-induced and age-associated memory impairments by the sigma and 5–hydroxytryptamine(1A) receptor agonist OPC-14523 (1-[3-[4-(3-chlorophenyl)-1-piperazinyl] propyl]-5-methoxy-3,4-dihydro-2[1H]-quinolinone monomethanesulfonate). J Pharmacol Exp Ther 301:249–257
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Nakamura, T., Komai, N., Isogami, I. et al. Memory and learning-enhancing effect of Daikenchuto, a traditional Japanese herbal medicine, in mice. J Nat Med 60, 64–67 (2006). https://doi.org/10.1007/s11418-005-0012-4
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DOI: https://doi.org/10.1007/s11418-005-0012-4