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Identification of c-Fos as a mitotic phosphoprotein: regulation of c-Fos by Aurora-A

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Journal of Biomedical Science

Abstract

The c-Fos has been implicated in the regulation of gene expression under a variety of stimuli. It is known that c-Fos undergoes protein phosphorylation, which may subsequently modulate diverse functions in cells. However, less is known about the role and phosphorylation status of c-Fos during mitosis. Here, we showed that c-Fos exhibited an electrophoretic mobility up-shift as detected by SDS-PAGE during mitosis, which is an indication of protein phosphorylation. Aurora-A, but not Aurora-B or -C, serves as one of the kinases catalyzing the mitotic phosphorylation of c-Fos. The mobility up-shift was partially abolished by introducing siRNA or a catalytically inactive form of Aurora-A. Moreover, ectopic expression of the wild type, but not the catalytically inactive form of Aurora-A resulted in the alteration of c-Fos complex formation, suggesting Aurora-A is engaged in the regulation of c-Fos protein–protein interaction. These findings imply that c-Fos may undergo cell cycle dependent phosphorylation, in which some kinases including Aurora-A play a role in catalyzing the post translational modification of c-Fos.

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Authors and Affiliations

  1. Graduate Institute of Biomedicine and Biomedical Technology, National Chi Nan University, Puli, Nantou, 545, Taiwan, R.O.C

    Chang-Tze Ricky Yu

  2. Department of Education and Research, Taichung Veterans General Hospital, Taichung, 400, Taiwan, R.O.C

    Jiunn-Chyi Wu & Shih-Lan Hsu

  3. Institute of Cancer Research, National Health Research Institutes, Taipei, 114, Taiwan, R.O.C

    Mei-Chih Liao & Chi-Ying F. Huang

  4. Institute of Clinical Medicine, National Yang-Ming University, Taipei, 112, Taiwan, R.O.C

    Chi-Ying F. Huang

  5. Institute of Bio-Pharmaceutical Sciences, National Yang-Ming University, Taipei, 112, Taiwan, R.O.C

    Chi-Ying F. Huang

  6. Institute of Biotechnology in Medicine, National Yang-Ming University, Taipei, 112, Taiwan, R.O.C

    Chi-Ying F. Huang

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  1. Chang-Tze Ricky Yu
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  2. Jiunn-Chyi Wu
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Correspondence to Chi-Ying F. Huang.

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Yu, CT.R., Wu, JC., Liao, MC. et al. Identification of c-Fos as a mitotic phosphoprotein: regulation of c-Fos by Aurora-A. J Biomed Sci 15, 79–87 (2008). https://doi.org/10.1007/s11373-007-9209-8

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  • DOI: https://doi.org/10.1007/s11373-007-9209-8

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