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Incorporation of dengue virus replicon into virus-like particles by a cell line stably expressing precursor membrane and envelope proteins of dengue virus type 2

Journal of Biomedical Science volume 15, pages 15–27 (2008)Cite this article

Abstract

While virus-like particles (VLPs) containing subgenomic replicons, which can transduce replicons into target cells efficiently for studying viral replication and vectors of gene therapy and vaccine, have been established for several flaviviruses, none has been reported for the four serotypes of dengue virus, the causal agent of the most important arboviral diseases in this century. In this study, we successfully established a cell line stably expressing the precursor membrane/envelope (PrM/E) proteins of dengue virus type 2 (DENV2), which can package a DENV2 replicon with deletion of PrM/E genes and produce single-round infectious VLPs. Moreover, it can package a similar replicon of different serotype, dengue virus type 4, and produce infectious chimeric VLPs. To our knowledge, this study reports for the first time replicon-containing VLPs of dengue virus. Moreover, this convenient system has potential as a valuable tool to study encapsidation of dengue virus and to develop novel chimeric VLPs containing dengue virus replicon as vaccine in the future.

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Acknowledgments

We thank Dr. G. J. J. Chang at the Center for Disease Control and Prevention, Fort Collins for kindly providing the plasmid, pCBD2-2J-2-9-1, Dr. C. J. Lai at the National Institute of Health for the plasmid p2AXhoI, Dr. S. S. Whitehead at the National Institute of Health for the plasmids p2 and p4, Dr. H. C. Wu at the Institute of Cellular and Organismic Biology, Academia Sinica, Taipei, Taiwan for the Mab DB29-1. This work was supported by the National Science Council Taiwan (NSC95-2320-B-002-084-MY3).

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Affiliations

  1. Institute of Microbiology, College of Medicine, National Taiwan University, No.1 Sec.1 Jen-Ai Rd, Taipei, Taiwan

    Chih-Yun Lai, Hsien-Ping Hu & Wei-Kung Wang

  2. Institute of Epidemiology, College of Public Health, National Taiwan University, Taipei, Taiwan

    Chwan-Chuen King

  3. Department of Internal Medicine, College of Medicine, National Taiwan University, Taipei, Taiwan

    Wei-Kung Wang

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  1. Chih-Yun Lai
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  2. Hsien-Ping Hu
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  3. Chwan-Chuen King
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  4. Wei-Kung Wang
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Correspondence to Wei-Kung Wang.

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Lai, CY., Hu, HP., King, CC. et al. Incorporation of dengue virus replicon into virus-like particles by a cell line stably expressing precursor membrane and envelope proteins of dengue virus type 2. J Biomed Sci 15, 15–27 (2008). https://doi.org/10.1007/s11373-007-9204-0

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  • DOI: https://doi.org/10.1007/s11373-007-9204-0

Keywords

  • dengue virus (DENV)
  • replicon
  • virus-like particles (VLPs)