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Neuroprotection and free radical scavenging effects of Osmanthus fragrans

Journal of Biomedical Science volume 14, pages 819–827 (2007)Cite this article

Abstract

The ethanol extract of dried flowers Osmanthus fragrans (OFE) was assessed for free radical scavenging effects measured by the bleaching of the 1,1-diphenyl-2-picryl-hydrazyl (DPPH) radical, scavenging of the hydroxyl anion, investigation of the ferric reducing/antioxidant power (FRAP) and lipid-peroxidation inhibition in rat tissues. OFE contained a high amount of total flavonoid and polyphenol. OFE presented the effects in the metal reducing power, FRAP assay with IC50 values of 0.23 µg/ml, and 7.74 µg/ml, respectively. OFE presented similar activities toward the DPPH and hydroxyl anion scavenging ability with IC50 values of 10 µg/ml. OFE with IC50 values between 46 and 97 µg/ml inhibited lipid peroxidation initiated by ferrous chloride in rat brain, liver, heart and kidney mitochodrias. Moreover, the neuroprotective activity of OFE was investigated under different insults (glutamate, arachidonic acid, and 6-hydroxydopamine) in Wistar rat primary cortical neurons. OFE with EC50 values between 66 and 165 µg/ml attenuated the neurotoxicity on MTT and LDH assays. In addition, the AKT protein expression of excitotoxicity and oxidative stress was displayed by western blotting analysis. OFE could up-regulate the glutamate and 6-OHDA decreased AKT expression. This is the first demonstration of the neuroprotective, free radical scavenging and anti-oxidative effects of O. fragrans.

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Authors and Affiliations

  1. Department of Pharmacognosy, School of Pharmacy, College of Pharmacy, Taipei Medical University, 250 Wu-Hsing Street, Taipei, 110, Taiwan

    Hsin-Hsueh Lee, Chi-Ting Lin & Ling-Ling Yang

  2. Topnotch Stroke Research Center, Taipei Medical University, No. 250, Wu-Hsing Street, Taipei City, 110, Taiwan

    Ling-Ling Yang

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  1. Hsin-Hsueh Lee
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  2. Chi-Ting Lin
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  3. Ling-Ling Yang
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Correspondence to Ling-Ling Yang.

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Lee, HH., Lin, CT. & Yang, LL. Neuroprotection and free radical scavenging effects of Osmanthus fragrans . J Biomed Sci 14, 819–827 (2007). https://doi.org/10.1007/s11373-007-9179-x

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  • DOI: https://doi.org/10.1007/s11373-007-9179-x

Keywords

  • Osmanthus fragrans
  • Free radical scavenging
  • Oxidative stress
  • Neuroprotection
  • Primary cortical neuron