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Neuroprotection and free radical scavenging effects of Osmanthus fragrans

  • Published:
Journal of Biomedical Science

Abstract

The ethanol extract of dried flowers Osmanthus fragrans (OFE) was assessed for free radical scavenging effects measured by the bleaching of the 1,1-diphenyl-2-picryl-hydrazyl (DPPH) radical, scavenging of the hydroxyl anion, investigation of the ferric reducing/antioxidant power (FRAP) and lipid-peroxidation inhibition in rat tissues. OFE contained a high amount of total flavonoid and polyphenol. OFE presented the effects in the metal reducing power, FRAP assay with IC50 values of 0.23 µg/ml, and 7.74 µg/ml, respectively. OFE presented similar activities toward the DPPH and hydroxyl anion scavenging ability with IC50 values of 10 µg/ml. OFE with IC50 values between 46 and 97 µg/ml inhibited lipid peroxidation initiated by ferrous chloride in rat brain, liver, heart and kidney mitochodrias. Moreover, the neuroprotective activity of OFE was investigated under different insults (glutamate, arachidonic acid, and 6-hydroxydopamine) in Wistar rat primary cortical neurons. OFE with EC50 values between 66 and 165 µg/ml attenuated the neurotoxicity on MTT and LDH assays. In addition, the AKT protein expression of excitotoxicity and oxidative stress was displayed by western blotting analysis. OFE could up-regulate the glutamate and 6-OHDA decreased AKT expression. This is the first demonstration of the neuroprotective, free radical scavenging and anti-oxidative effects of O. fragrans.

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Authors and Affiliations

  1. Department of Pharmacognosy, School of Pharmacy, College of Pharmacy, Taipei Medical University, 250 Wu-Hsing Street, Taipei, 110, Taiwan

    Hsin-Hsueh Lee, Chi-Ting Lin & Ling-Ling Yang

  2. Topnotch Stroke Research Center, Taipei Medical University, No. 250, Wu-Hsing Street, Taipei City, 110, Taiwan

    Ling-Ling Yang

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  1. Hsin-Hsueh Lee
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  2. Chi-Ting Lin
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Correspondence to Ling-Ling Yang.

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Lee, HH., Lin, CT. & Yang, LL. Neuroprotection and free radical scavenging effects of Osmanthus fragrans . J Biomed Sci 14, 819–827 (2007). https://doi.org/10.1007/s11373-007-9179-x

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  • DOI: https://doi.org/10.1007/s11373-007-9179-x

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